pH对棘胸蛙蝌蚪若干生理指标的影响效应

发布时间：2018-03-29 05:06

  本文选题：pH　切入点：棘胸蛙蝌蚪　出处：《浙江海洋大学》2017年硕士论文

【摘要】：以1月龄棘胸蛙蝌蚪[体长(1.694±0.121)cm,体质量(0.548±0.062)g]为实验材料,以经自然曝气48h自来水[水温(24±0.2)℃、pH7.30±0.01、DO(7.30±0.01)mg/L]为实验水源,设置5.5、6.5、7.0、7.5、8.5和9.5等6个pH处理组,在静水停饲的条件下,观察pH对蝌蚪行为活动和尾部皮肤色差Hunter L、A、B值影响的同时,较系统开展了pH对其耗氧率、排氨率和窒息点的影响,以及胁迫和恢复条件下棘胸蛙蝌蚪肝脏抗氧化酶(SOD、CAT、GSH)以及尾部皮肤ATP酶和抗氧化酶(SOD、CAT)活力的变化特征。结果表明:(1)蝌蚪分布水层、集群状况和运动频率与其所处pH值关系紧密,pH 5.5-6.5实验组棘胸蛙蝌蚪分布于水体上层且呈分散特征,且伴有浮头现象;pH 7.0实验组和pH9.5实验组棘胸蛙蝌蚪分布整个水体,且伴有浮头现象;pH 7.5-8.5实验组棘胸蛙蝌蚪分布于整个水体且呈集群特征;pH 5.5-7.0实验组蝌蚪运动频率明显高于其他实验组;(2)L值可表示蝌蚪尾部皮肤色差感应pH的指标,pH 8.5时L值最小,表明pH 8.5为显著改变蝌蚪皮肤色差的临界值;(3)pH 7.5-9.5时的耗氧率在各时段及夜均、昼均和日均均无显著差异(P0.05),且显著高于其余组别(P0.05),故pH 7.5为蝌蚪表露正常耗氧特征的最低临界值;各实验组排氨昼夜节律变化均呈夜均昼均(P0.05),且其日均排氨率与夜均、昼均排氨率均无显著差异(P0.05),即蝌蚪排氨率在pH胁迫下未受到影响;窒息点含氧量随pH的升高呈先下降后上升的趋势,且其pH 7.5时窒息点含氧量最低;(4)pH 7.5为蝌蚪肝脏SOD和CAT酶耐受pH胁迫的最适值,pH 8.5为最高临界值,且恢复时长仅需6d可恢复到pH7.0水平;GSH酶在pH 7.5时未受到胁迫。(5)pH 7.5为蝌蚪尾部皮肤SOD和CAT酶耐受pH胁迫的最适值,pH 8.5为最高临界值,且恢复时长仅需6d可恢复到pH7.0水平;ATP酶在pH 7.5时未受到胁迫。
[Abstract]:The tadpoles (1.694 卤0.121g) and 0.548 卤0.062g (body weight) were used as experimental materials. The water was exposed to natural aeration for 48h [pH 7.30 卤0.01DO 7.30 卤0.01)mg/L]. Six pH groups (5.56.57.07.07.5g and 9.5) were set up under the condition of stopping feeding in static water. The effects of pH on tadpoles' behavior and tail skin color difference (Hunter) were observed. The effects of pH on oxygen consumption rate, ammonia excretion rate and asphyxiation point were carried out systematically. The changes of the activities of liver antioxidant enzyme SODCATH and tail skin ATP enzyme and antioxidant enzyme SOD CAT in tadpoles of Rana spinothornica under stress and recovery were also studied. The results showed that the tadpoles were distributed in water layer. The tadpoles of the experimental group were distributed in the upper layer of the water body with the characteristics of dispersion, and the tadpoles of the experimental group were distributed throughout the water body with floating head phenomenon. The tadpoles of the experimental group and the pH9.5 experimental group were distributed throughout the water body. The tadpoles of the experimental group with floating head phenomenon and pH 7.5-8.5 were distributed in the whole water body and showed cluster characteristics. The tadpoles of the experimental group with pH 5.5-7.0 were significantly higher than those of the other experimental groups. The results indicated that the tadpole tadpoles had the lowest tadpole tadpole tadpole tail skin color difference induced pH at pH 8.5, and the tadpole movement frequency of the experimental group was significantly higher than other experimental groups. The results showed that pH 8.5 was the critical value to change the color difference of tadpole skin significantly. When pH value was 7.5-9.5, the oxygen consumption rate of tadpoles had no significant difference at each time and night, day and day, and was significantly higher than that of other groups, so pH 7.5 was the lowest critical value for tadpoles to show normal oxygen consumption. The circadian rhythm of ammonia excretion in each experimental group was P0.05a, and the average daily ammonia excretion rate was not significantly different from that of night-average ammonia excretion rate, that is, the ammonia excretion rate of tadpoles was not affected by pH stress. The oxygen content of asphyxiated point decreased first and then increased with the increase of pH value, and the lowest oxygen content in asphyxiating point was the lowest at pH 7.5. Ph 7.5 was the most suitable value for SOD and CAT enzyme tolerance to pH stress in tadpole liver and pH 8.5 was the highest critical value. It only took 6 days to recover to pH7.0 level. When pH 7.5 was not under stress, pH 7.5 was the most suitable value for tadpole tail skin SOD and CAT enzyme tolerance to pH stress, and pH 8.5 was the highest critical value for tadpole tail skin SOD and CAT enzyme tolerance to pH stress. The recovery time was only 6 days, and the pH7.0 level could be recovered without stress at pH 7. 5.
【学位授予单位】：浙江海洋大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S917.4

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