8个紫花苜蓿品种抗旱、抗寒性分析研究

发布时间：2018-03-29 14:33

  本文选题：紫花苜蓿　切入点：抗旱性　出处：《西北农林科技大学》2017年硕士论文

【摘要】：紫花苜蓿(MedicagosativaL.)是我国最重要的豆科牧草之一,其抗逆性的研究对苜蓿品种的选择和布局至关重要。本研究通过对新牧2、三得利、赛迪5、WL-656、WL-168、WL-903、WL-712、WL-343的抗旱性、抗寒性进行初步的分析,拟为引进品种在我国进一步的栽培利用提供科学依据。研究结果如下:1.通过10%PEG模拟干旱处理,采用水培法研究8个苜蓿品种的抗旱性,测定其干旱胁迫后植物体的根长、株高、鲜干比、游离脯氨酸含量和相对含水量五个抗早指标。结果如下:在干旱胁迫下,8个苜蓿品种幼苗根系缩短(8.87%-29.40%)、株高减少(3.77%-15.89%),差异显著(p0.05),且处理组脯氨酸含量与对照组相比呈上升趋势(21.24%-39.04%),差异显著(p0.05),干旱胁迫下苜蓿体内生物量不断累积,从而表现为鲜干比值降低(24.51%-37.81%)与相对含水量下降(13.31%-16.71%),差异显著(p0.05)。将8个苜蓿品种的抗旱性进行排序,其中抗旱性最强的是WL-168,最弱的为三得利,其余品种抗旱性强弱依次为:新牧2、WL-712、赛迪5、WL-343、WL-656、WL-903。2.通过4℃低温处理苜蓿幼苗(具有三片真叶),在3d、5d、7d分别测定其低温胁迫后植物体的根长、株高、鲜干比和相对电导率四个抗寒指标。研究表明:8个苜蓿品种幼苗根系缩短(5.96%-21.51%)、株高降低(5.56%-15.88%),差异显著(p0.05),鲜干比显著下降(21.18%-36.64%)(p0.05),由于低温对细胞膜的损害导致处理组的相对电导率与对照组的相比显著上升,增加了(20.94%-37.50%)(p0.05)。将8个苜蓿品种的抗寒性进行排序,其中抗寒性最强的是赛迪5,最弱的为WL-903,其余品种抗寒性强弱依次为:WL-168、WL-712、新牧2、WL-343、三得利、WL-656。本论文通过对8个苜蓿品种抗旱性和抗寒性的研究,初步研究结果表明:抗旱性和抗寒性都较强的品种都为WL-168、WL-712和赛迪5。研究结果可为引进品种在我国的栽培种植提供一定科学依据。
[Abstract]:Medicago sativa L.) is one of the most important legume forages in China, and its resistance to stress is very important to the selection and distribution of alfalfa varieties. To provide scientific basis for further cultivation and utilization of introduced varieties in China. The results are as follows: 1. The drought resistance of 8 alfalfa varieties was studied by 10%PEG simulated drought treatment, and the root length and plant height of 8 alfalfa varieties after drought stress were measured. Fresh / dry ratio, The results are as follows: under drought stress, the root system of 8 alfalfa varieties was shortened by 8.87-29.40m and the plant height decreased by 3.77- 15.890.The difference was significant (p 0.05), and the proline content of the treatment group was higher than that of the control group. The increasing trend was 21.24- 39.040.The difference was significant (p0.05). Under drought stress, the biomass of alfalfa accumulated continuously. The results showed that the ratio of fresh and dry decreased by 24.51-37.81) and the relative water content decreased by 13.31- 16.71.The drought resistance of 8 alfalfa varieties was ranked according to the order of drought resistance, among which WL-168 was the most drought-resistant and the weakest was Sanreji. The drought resistance of the other varieties was as follows: Xinmu 2WL-712, Seidi 5WL-343WL-656 WL-903.2.The alfalfa seedlings (with three true leaves) were treated at 4 鈩，

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