盐穗木正丁醇萃取物对金黄色葡萄球菌生长的影响

发布时间：2018-04-03 00:17

本文选题：盐穗木　切入点：正丁醇萃取物　出处：《塔里木大学》2017年硕士论文

【摘要】：盐穗木(Halostachys caspica)是荒漠主要的植物之一,是隶属于藜科植物,对盐土地有较为良好的耐受性。早年已有研究发现盐穗木的正丁醇萃取物对金黄色葡萄球菌有较好的抑制生长的效果,因而本实验通过MIC和MBC的测定、St.aureus生长曲线的测定、纤维结合蛋白试验、电导率测定法、培养液中蛋白质浓度测定、培养液中还原糖浓度测定、β-半乳糖苷酶活力测定法、碱性磷酸酶测定、胞内Ca2+浓度测定等,测定并比较不同溶剂盐穗木萃取物的抑菌活性进行;为明确金黄色葡萄球菌细胞膜的渗透性,我们测定了金黄色葡萄球细胞内抗氧化酶酶活性测定、金黄色葡萄球菌酶液粗测定、金黄色葡萄球菌细胞内源H2O2含量的测定、金黄色葡萄球菌细胞脂质过氧化测定;最后,明确盐穗木正丁醇萃取物对金黄色葡萄球菌细胞形态改变的影响。试验研究得到如下结果:1、盐穗木含有多种提取的化学成分,其中主要包括糖、多糖、多肽、氨基酸、有机酸、甾体、三萜类物质、皂苷、酚类、蹂质、生物碱、黄酮、挥发油等;盐穗木正丁醇萃取物对金黄色葡萄球菌生长曲线的影响,主要体现在金黄色葡萄球菌生长的对数时期;同时,对金黄色葡萄球菌,盐穗木正丁醇萃取物的抑菌效果较为良好,其最低杀菌浓度(MBC)为0.1250 mg/mL,最小抑菌浓度(MIC)为0.0625 mg/mL,且比对照组提前7h进入稳定期。2、含有正丁醇萃取物的药液经100℃处理1h,抑菌率80%,说明该活性物质的热稳定性较好,可配合高温设备的清洗杀菌。正丁醇萃取物在pH值7-8环境中所具有的抑菌活性最高,抑菌率90%,有望用于容易感染金黄色葡萄球菌的各种肉类、水产、蛋类等pH值中性食品的防腐保鲜。3、正丁醇萃取物所致的细胞膜通透性的改变,会引起溶液外物质浓度、种类、胞外电导率的变化,这将引起细胞内钾钠离子等小分子物质的外漏,当细胞膜破坏严重时,将造成永久性伤害,这时会出现胞内蛋白质等大分子物质漏到胞外基质中,随着效果时间的延长,细胞膜将被正丁醇萃取完全破坏,培养液电导率将胞内物质释放到胞外基质快速上升。
[Abstract]:Halostachys caspicia is one of the main plants in desert and belongs to the family Chenopodiaceae. Halostachys caspicia has good tolerance to salt soil.In early years, it was found that n-butanol extract from salt-spikelet had a good inhibitory effect on the growth of Staphylococcus aureus. Therefore, the growth curve of St.aureus was determined by MIC and MBC, fibronectin test and electrical conductivity test were used to determine the growth curve of Staphylococcus aureus.Protein concentration in culture medium, reducing sugar concentration in culture medium, 尾 -galactosidase activity, alkaline phosphatase and intracellular Ca2 concentration were measured and compared.In order to determine the permeability of staphylococcus aureus cell membrane, we measured the activity of antioxidant enzymes in the cells of grape ball, the crude enzyme solution of Staphylococcus aureus, and the content of endogenous H2O2 in the cells of Staphylococcus aureus.Determination of lipid peroxidation of Staphylococcus aureus cells. Finally, the effect of n-butanol extract from Staphylococcus aureus on the morphological changes of Staphylococcus aureus cells was determined.The results of the experiment are as follows: 1: 1. There are many kinds of chemical constituents, including sugar, polysaccharides, polypeptides, amino acids, organic acids, steroids, triterpenes, saponins, phenols, ragged substances, alkaloids, flavonoids, etc.The effect of n-butanol extract on the growth curve of Staphylococcus aureus is mainly reflected in the logarithmic period of the growth of Staphylococcus aureus.The antimicrobial effect of n-butanol extract from salt-panicle was good.It can be combined with the cleaning and sterilization of high temperature equipment.The n-butanol extract has the highest bacteriostatic activity in pH 7-8 environment, and the bacteriostatic rate is 90%, which is expected to be used in various kinds of meat and aquatic products susceptible to infection with Staphylococcus aureus.The change of cell membrane permeability caused by n-butanol extract may cause the change of extracellular concentration, species and extracellular conductivity of neutral food with pH value such as eggs.This will cause leakage of small molecules such as potassium, sodium ions and other small molecules in the cell. When the cell membrane is seriously damaged, it will cause permanent damage. At this time, macromolecular substances such as intracellular proteins will leak into the extracellular matrix, and with the prolongation of the effect time,The membrane was completely destroyed by n-butanol extraction, and the electrical conductivity of the medium released the intracellular substance to the extracellular matrix.
【学位授予单位】：塔里木大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S853.7

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