金丝草抑菌活性成分研究

发布时间：2018-08-16 11:52

【摘要】：为探索金丝草Pogonatherum crinitum在植物病原菌防治方面的应用前景,测定了金丝草水提取物及其乙酸乙酯、正丁醇萃取物对20种常见农作物病原真菌的抑菌活性。结果表明,2.00×10~3 mg/L的乙酸乙酯萃取物对其中8种植物病原菌的抑制率大于50%,尤其对辣椒疫霉Phytophthora capsici的抑制率高达100%。由乙酸乙酯萃取物进一步分离得到10个已知化合物,经波谱学方法鉴定其分别为1,2,4-三羟基苯(1)、1,2-二羟基-4-甲氧基苯(2)、小麦黄素-7-O-β-D-吡喃葡萄糖苷(3)、小麦黄素(4)、β-腺苷(5)、咖啡酸(6)、留兰香木脂素B(7)、木犀草素6-C-β-波依文糖-7-O-β-葡萄糖(8)、芦丁(9)及槲皮素(10),其中化合物2、3、5、7和10为从该植物中首次分离得到。孢子萌发法测定结果表明:化合物6~10对棉花立枯丝核菌Rhizoctonia solani和辣椒疫霉的孢子萌发具有较好的抑制作用,其EC_(50)值分别在18.54~103.6 mg/L和21.38~87.83 mg/L之间。
[Abstract]:In order to explore the application prospect of Pogonatherum crinitum in the prevention and control of plant pathogens, the antimicrobial activity of water extract, ethyl acetate and n-butanol extract against 20 common crop pathogenic fungi was determined. The results showed that the inhibitory rate of ethyl acetate extract of 2.00 脳 10 ~ (-3) mg/L on 8 plant pathogens was more than 50%, especially on Phytophthora capsici Phytophthora capsici. Ten known compounds were further isolated from ethyl acetate extract. They were identified by spectroscopic method as 1tandruff 4-trihydroxylbenzene (1), trihydroxy-2-dihydroxy-4-methoxy benzene (2), wheatflavin -7-O- 尾 -Dpyranoside (3), wheatflavin (4), 尾 -adenosine (5), caffeic acid (6), tenella lignin B (7), luteolin 6-C- 尾 -bovinose -7-O- 尾 -grape. Sugar (8), rutin (9) and quercetin (10) were isolated from the plant for the first time. The results of spore germination test showed that compound 6Y10 could inhibit the spore germination of Rhizoctonia solani Rhizoctonia solani and Phytophthora capsici, and its EC50 values were between 18.54 ~ 103.6 mg/L and 21.38 ~ 87.83 mg/L, respectively.
【作者单位】：福建农林大学植物保护学院;
【分类号】：S481

【参考文献】