干旱胁迫下葡萄超微弱发光的变化及其发生机制

发布时间：2018-10-12 08:11

【摘要】：试验以赤霞珠和梅鹿辄为材料,采用控水方式进行干旱胁迫处理,通过研究两种葡萄在干旱胁迫过程中超微弱发光、生长活力、光合作用、活性氧系统以及能量水平的变化,来探讨在干旱胁迫过程中,葡萄叶片超微弱发光的变化以及与光合作用、活性氧以及能量水平等的关系,研究主要结果如下:1.在干旱胁迫过程中,两种葡萄的土壤含水量、叶片相对含水量、自由水含量、自由水/束缚水比值、根系活力均逐渐下降,束缚水含量逐渐上升,即葡萄的生长活力降低。UWL强度均呈下降趋势,说明在干旱胁迫下,UWL强度随胁迫程度的加重而下降,UWL可以反映葡萄受胁迫的程度。2.在干旱胁迫过程中,两种葡萄叶片的活性氧均呈现上升趋势,UWL强度均呈下降趋势,说明活性氧的累积并没有引起UWL强度的增加,活性氧并不是UWL的直接来源。3.在干旱胁迫过程中,两种葡萄叶片的光合参数Pn值、Tr值、Gs值逐渐下降,Ci值逐渐上升;荧光参数Fv/Fm、Yield、qP逐渐下降、qN逐渐上升;叶绿素a和叶绿素b含量均呈下降趋势,UWL强度呈下降趋势。说明在干早胁迫过程中,UWL强度随光合作用的减弱而降低,光合作用与UWL的产生有关。4.在干旱胁迫过程中,两种葡萄叶片的ATP含量、能荷值呈下降趋势,ADP、AMP含量呈上升趋势,UWL强度呈下降趋势。说明在干旱胁迫过程中,UWL强度随能量水平的减弱而降低,能量是UWL的主要来源之一。
[Abstract]:Using Cabernet Sauvignon and Merlot as materials, the changes of ultraweak luminescence, growth vigor, photosynthesis, active oxygen system and energy level of two grape varieties during drought stress were studied. To study the changes of ultraweak luminescence in grape leaves during drought stress and its relationship with photosynthesis, reactive oxygen species and energy levels. The main results are as follows: 1. During drought stress, the soil water content, leaf relative water content, free water / bound water ratio, root activity and bound water content of the two grape varieties decreased gradually, and the bound water content increased gradually. That is to say, the growth activity of grape decreased and the UWL intensity decreased, indicating that the UWL intensity decreased with the increase of stress degree under drought stress, and UWL could reflect the degree of grape stress. 2. In the course of drought stress, the active oxygen species of both grape leaves showed an upward trend, and the intensity of UWL decreased, indicating that the accumulation of active oxygen species did not cause the increase of UWL intensity, and the active oxygen species were not the direct source of UWL. 3. During drought stress, the photosynthetic parameters (Pn, Tr, Gs, Ci) of the two grape leaves gradually decreased, while the fluorescence parameter Fv/Fm,Yield,qP decreased and qN gradually increased. The content of chlorophyll a and chlorophyll b decreased and the intensity of UWL decreased. The results showed that the intensity of UWL decreased with the decrease of photosynthesis during dry morning stress, and photosynthesis was related to the production of UWL. During drought stress, the ATP content, energy charge value, ADP,AMP content and UWL intensity of the two grape leaves showed a decreasing trend, an increasing trend, and a decreasing trend of UWL intensity. The results showed that the intensity of UWL decreased with the decrease of energy level during drought stress, and energy was one of the main sources of UWL.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S663.1

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