外源氨基酸对Bt棉杀虫蛋白含量影响及蛋白质周转机制

发布时间：2021-10-09 05:20

　　转Bt基因抗虫棉生殖器官杀虫蛋白低表达量影响抗虫性。为提高生殖器官蕾花铃杀虫蛋白的表达,以常规品种泗抗1号（s1）和杂交品种泗抗3号（s3）为试验材料,分别于2016年、2017年和2018年在以前研究基础上设计构成Bt蛋白主要成份的5种氨基酸和所有21种氨基酸处理喷洒蕾花铃探讨氨基酸外用对Bt棉生殖器官杀虫蛋白表达量影响及相关的蛋白质周转机制。研究结果表明:与对照相比,两个氨基酸处理均显著提高了蕾的Bt蛋白含量,Bt蛋白含量增加7.4%-19.4%,全氨基酸应用对棉蕾Bt蛋白含量提高的幅度更大主要与蛋白质降解酶蛋白酶和肽酶活性下降幅度更大有关。氨基酸处理显著提高花中Bt蛋白含量,比对照提高了15.2%～25.8%。但两个氨基酸处理间差异不显著。相似地,2个氨基酸处理的铃壳、棉子和纤维中Bt蛋白也显著提高。如花后25天,泗抗1号Bt蛋白含量量铃壳中提高了 7.4%%-9.30%、棉子中高了23.9%-71.1%,纤维中提高了 23.8%-172.0%;泗抗3号Bt蛋白含量量铃壳中提高了39.0-41.3%、棉子中提高了 15.8%-53.8%、纤维中提高了 20.0%-230.3%。此... 

【文章来源】：扬州大学江苏省

【文章页数】：50 页

【学位级别】：硕士

【文章目录】：
Abstract
中文摘要
1. Introduction
2. Materials and Methods
    2.1 Materials and experimental design
    2.2 Preparation of plant material
        2.2.1 Sampling
            2.2.1.1 The cry IAc protein content
            2.2.1.2 Free amino acid and soluble protein content
            2.2.1.3 Glutamic-pyruvic transaminase (GPT) and glutamate oxaloacetatetransaminase (GOT)
            2.2.1.4 Protease and peptidase activity
    2.3 Statistics analysis
3. Results
    3.1 The amino acids application treatments on the square insecticidal property
        3.1.1 On the square insecticidal protein concentration
        3.1.2 The amino acids application treatments on square nitrogen metabolismcharacteristics
            3.1.2.1 On square GPT and GOT activity
            3.1.2.3 On the square amino acid and soluble protein content
        3.1.3 Relationship between the square insecticidal protein concentration and nitrogenmetabolism
    3.2 The amino acids application treatments on the flower insecticidal property
        3.2.1 On the flower Bt protein concentration
        3.2.2 On the flower nitrogen metabolism characteristics
            3.2.2.1 On flower GPT and GOT activity
            3.2.2.2 On flower protease and peptidase activity
            3.2.2.3 On the flower amino acid and soluble protein content
        3.2.3 Relationship between the flower insecticidal protein concentration and nitrogenmetabolism
    3.3 The amino acids application treatments on the boll insecticidal property
        3.3.1 On the boll shell insecticidal property
            3.3.1.1 on boll shell Bt protein concentration
            3.3.1.2 on boll shell nitrogen metabolism characteristics
                3.3.1.2.1 On boll shell GPT and GOT activity
                3.3.1.2.2 On boll shell protease and peptidase activity
                3.3.1.2.3 On the boll shell amino acid and soluble protein content
            3.3.1.3 Relationship between the boll shell insecticidal protein concentration andnitrogen metabolism
        3.3.2 The amino acids application treatments on the seeds insecticidal property
            3.3.2.1 On the Cotton seeds insecticidal protein concentration
            3.3.2.2 on seeds nitrogen metabolism characteristics
                3.3.2.2.1 On the seeds GPT and GOT activity
                3.3.2.2.2 On seeds protease and peptidase activity
                3.3.2.2.3 On the seeds amino acid and soluble protein content
            3.3.2.3 Relationship between the seeds insecticidal protein concentration andnitrogen metabolism
        3.3.3 The amino acids application treatments on the fiber insecticidal property
            3.3.3.1 On fiber insecticidal protein concentration
                3.3.3.1.1 On seeds GPT and GOT activity
                3.3.3.1.2 On fiber protease and peptidase activity
                3.3.3.1.3 On fiber soluble protein and amino acid content
            3.3.3.2 Relationship between the fiber insecticidal protein concentration andnitrogen metabolism
4. Discussion
    4.1 Amino acid application enhanced square, flower and boll Bt protein concentration in Btcotton
    4.2 Increased protein synthesis and reduced protein degradation by exterior amino acidapplication caused elevated Bt toxin content in Bt cotton
5. Conclusions
6. References
Acknowledgement
The publications of the author


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