猫轻型脑外伤模型的建立与影像学、病理学研究

发布时间：2018-07-08 14:07

本文选题：生物力学 + 颅脑损伤　；参考：《昆明医学院》2006年硕士论文

【摘要】：第一部分猫脑外伤动物模型研究目的探讨不同部位不同力量的猫脑外伤生物力学特点和颅脑损伤特点，找到用于研究轻型脑外伤的最佳动物模型。方法随机选择健康纯白色短毛3～4月家猫50只，体重0.8～0.9Kg，雌雄不限。按打击部位不同分组，额部组12只、左颞部组12只、顶部组12只、枕部组12只，正常对照组2只。按打击力量分组，又把每组分为不同力量六组。用改良单摆式颅脑损伤仪按不同部位不同力量分别对每只猫行一次性打击。打击后作行为学观察，观察相应的时间后麻醉处死解剖取脑，行病理学观察。结果额部组：在6.04J时出现颅骨骨折，但无昏迷和蛛网膜下腔出血；在10.11J时同时出现严重的昏迷、颅骨骨折和蛛网膜下腔出血；未见明显的脑挫裂伤。左颞部组和顶部组：两组脑损伤情况相似，在3.97J和6.04J颅脑损伤均不明显，7.76J、8.85J、10.11J、11.38J时同时出现严重的昏迷、颅骨骨折、蛛网膜下腔出血和不同程度的脑挫裂伤。枕部组：3.97～7.76J未出现颅脑损伤；8.85～10.11J出现短暂的昏迷，有或无线性骨折，，颅内损伤不明显；11.38J可能出现较重的颅脑损伤。结论本研究制作的改良单摆式颅脑损伤仪是较理想的猫颅脑损伤动物模型研究的实验仪器；纯白色短毛3～4月体重0.8～0.9Kg家猫适合颅脑损伤的研究；额、颞、顶部颅骨较枕部更易受损，不适合轻型脑外伤的研究；选择纯白色短毛3～4月体重0.8～0.9Kg家猫，使用改良单摆式颅脑损伤仪给予8.85～10.11J力量一次性扣击猫头枕部易建立理想的猫轻型脑外伤动物模型。
[Abstract]:The first part of the animal model of cat brain trauma objective to explore the biomechanical characteristics and brain injury characteristics of cat brain injury with different positions and forces, and to find the best animal model for the study of mild brain injury. Methods A total of 50 healthy white short-haired cats with a body weight of 0.80.9kg / g were randomly selected for 3 ~ 4 months. There were 12 rats in frontal group, 12 in left temporal group, 12 in parietal group, 12 in occipital group and 2 in normal control group. Each group is divided into six groups of different forces. The modified single pendulum craniocerebral injury instrument was used to attack each cat in one time according to different positions and forces. The behavior was observed after attack, and the brain was dissected after anaesthesia for the corresponding time, and the pathology was observed. Results in the frontal group, there were no coma and subarachnoid hemorrhage at 6.04J, severe coma at 10.11J, cranial fracture and subarachnoid hemorrhage, no obvious cerebral contusion and laceration. In the left temporal group and the parietal group, the condition of brain injury was similar. Severe coma, skull fracture, subarachnoid hemorrhage and different degrees of cerebral contusion occurred at the same time when the brain injury was not obvious at 3.97J and 6.04J. In the occipital group, there was no transient coma at 8.8510. 11J in the occipital group. The craniocerebral injury was not obvious in the occipital group and the craniocerebral injury was not obvious. Conclusion the modified single-pendulum craniocerebral injury instrument made in this study is an ideal experimental instrument for the study of animal model of craniocerebral injury in cats, pure white short hair with body weight of 0.80.9kg for 3 ~ 4 months is suitable for the study of craniocerebral injury. The parietal skull is more easily damaged than the occipital part and is not suitable for the study of mild brain trauma. It is easy to establish an ideal animal model of light brain injury in cats by using the modified single pendulum craniocerebral injury instrument with 8.85J 10.11J force once clasping the occipital part of the cat's head.
【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R651.1;R-332

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