下丘脑室旁核ATP敏感性钾离子通道参与大鼠炎性痛调节的作用研究

发布时间：2018-07-08 18:05

本文选题：ATP敏感性钾离子通道 + 下丘脑　；参考：《中国药理学通报》2014年09期

【摘要】：目的探讨下丘脑室旁核ATP敏感性钾离子通道(KATP通道)在大鼠炎性痛中的作用。方法♂SD大鼠(250~280 g),采用随机数字法分为5组(每组6只):正常组(Normal组)、完全弗氏佐剂致炎性痛组(CFA组)、生理盐水组(Saline组)、KATP通道特异性激动剂组(Diaoxide组)和激动剂溶媒组(Vehicle组)。以热痛敏刺激仪检测各组大鼠热缩足潜伏期(TWL),观察痛行为学变化;以免疫荧光技术观察室旁核KATP通道和脊髓背角c-Fos阳性细胞数的变化。并观察KATP通道激动剂对大鼠痛行为和脊髓背角c-Fos表达的影响。结果 1与术前和Saline组相比,CFA组大鼠炎症侧后足术后d 1、d 3和d 7的TWL降低(P0.05),CFA组术后d 3、d 7的KATP阳性细胞数减少(P0.01),脊髓背角c-Fos阳性细胞数增加(P0.01);2与Vehicle组相比,激动剂组大鼠热痛觉过敏减轻(P0.01),脊髓背角c-Fos阳性细胞数减少(P0.01)。结论下丘脑室旁核KATP通道可能与CFA所致炎性痛的发生机制相关。
[Abstract]:Objective to investigate the role of ATP sensitive potassium channel (K ATP channel) in hypothalamic paraventricular nucleus (PVN) in inflammatory pain in rats. Methods SD rats (250,280 g),) were randomly divided into 5 groups (6 rats in each group): normal group (normal group), complete Freund's adjuvant induced inflammatory pain group (CFA group), saline group (Saline group), KATP channel specific agonist group (Diaoxide group) and agonist solvent group (vehicle group). The latent period of thermal contraction foot (TWL) was detected by thermo-sensitive stimulator and the changes of pain behavior were observed. The changes of K ATP channels in paraventricular nucleus and c-Fos positive cells in dorsal horn of spinal cord were observed by immunofluorescence technique. The effects of K ATP channel agonist on pain behavior and c Fos expression in dorsal horn of spinal cord in rats were observed. Results (1) compared with the preoperative and Saline groups, the TWL levels in the CFA group were significantly lower than those in the control group (P0.05), and the number of KATP positive cells in the spinal dorsal horn (P0.01) and the number of c-Fos positive cells in the dorsal horn of spinal cord (P0.01) were significantly higher in the CFA group than in the vehicle group. In the agonist group, thermal hyperalgesia was alleviated (P0.01) and the number of c-Fos positive cells in the dorsal horn of spinal cord decreased (P0.01). Conclusion the K ATP channel in hypothalamic paraventricular nucleus may be related to the inflammatory pain induced by CFA.
【作者单位】：徐州医学院江苏省麻醉学重点实验室;嘉兴市第二医院麻醉科;
【基金】：国家自然科学基金资助项目(No 81371243) 江苏省自然科学基金资助项目(No BK2012580)
【分类号】：R363

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