蛋白质乙酰化对HBV复制的影响

发布时间：2017-12-27 09:17

本文关键词：蛋白质乙酰化对HBV复制的影响　出处：《临床肝胆病杂志》2016年08期 　论文类型：期刊论文

【摘要】：目的探讨宿主细胞蛋白质乙酰化修饰对HBV复制的影响。方法采用去乙酰化酶抑制剂制滴菌素A(TSA)和尼克酰胺(NAM)刺激HBV复制细胞模型Hep G2.2.15和Hep AD38,并检测细胞内的HBV复制指标。采用Western Blot检测广谱乙酰化蛋白和乙酰化组蛋白H3的表达水平。结果 TSA和NAM刺激细胞会引起细胞内蛋白质的乙酰化水平升高,且乙酰化修饰水平增高呈时间和浓度依赖的变化趋势。两种细胞受TSA和NAM刺激的影响,培养上清中的HBs Ag水平降低,而HBV DNA水平升高,且呈时间和浓度依赖关系,而两种细胞培养上清中的HBe Ag和细胞内核心颗粒DNA的表达水平变化趋势不明显。结论宿主蛋白质的乙酰化可能参与和影响了细胞内HBV的复制过程,进一步深入分析和鉴定影响HBV胞内复制的宿主乙酰化蛋白质将有助于加深对HBV复制调控的认识,为开发特异性抗病毒策略提供新思路。
[Abstract]:Objective to investigate the effect of protein acetylation on HBV replication in host cells. Methods using the deacetylase inhibitor trichostatin A (TSA) and nicotinamide (NAM) stimulated HBV replication cell model of Hep G2.2.15 and Hep AD38, and the detection of intracellular HBV replication. The expression level of broad-spectrum acetylation protein and acetyl group protein H3 was detected by Western Blot. Results TSA and NAM stimulated the level of acetylation of protein in cells, and the level of acetylation increased in a time and concentration dependent manner. Two kinds of cells were stimulated by TSA and NAM, and the level of HBs Ag in culture supernatant decreased, while the level of HBV DNA increased. It was time and concentration dependent. However, the expression level of HBe Ag and intracellular core particle DNA in two cell culture supernatants was not obvious. Conclusion the host protein acetylation may participate in and influence the replication of intracellular HBV, host protein acetylation further analysis and identification of HBV intracellular replication will contribute to the understanding of the regulation of HBV replication, and provide new ideas for the development of specific antiviral strategies.
【作者单位】：复旦大学附属公共卫生临床中心;
【基金】：上海市自然科学基金青年项目(13ZR1460100) 国家自然科学基金(81271834) 国家863课题(2014AA021403)
【分类号】：R512.62
【正文快照】： 中国是HBV感染的高发区,约有1亿HBV携带者[1-3]。宿主细胞基因组编码蛋白质的乙酰化修饰,在调控胞内HBV复制过程中发挥多重作用。因此,探讨细胞内蛋白质乙酰化水平的改变对病毒复制指标的影响,对深入了解乙酰化修饰对HBV复制的调控作用具有重要意义。1材料与方法1.1细胞株与试

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