H3N2亚型流感病毒分离株全基因组序列特征分析及其临床意义

发布时间：2018-01-16 05:32

本文关键词：H3N2亚型流感病毒分离株全基因组序列特征分析及其临床意义　出处：《上海交通大学学报(医学版)》2015年11期 　论文类型：期刊论文

【摘要】：目的分析H3N2亚型流感病毒流行株的全基因组序列及其与临床特征的相关性。方法检测2014年12月—2015年3月收集的呼吸道感染患者鼻拭子样本,对其中H3N2亚型流感病毒株PCR扩增后直接进行全基因组测序,分析其核酸序列特征及氨基酸变异情况。结果与WHO推荐2014—2015流感疫苗株参考病毒株相比,来自不同患者的14个病毒株HA1蛋白抗原决定簇均发生不同程度突变,抗原决定簇结构改变较明显的病毒株其宿主具有更高的体温和更严重的临床表现。全部14个病毒株发生HA1蛋白E190D氨基酸突变。6个病毒株NA蛋白出现1个新增糖基化位点。1个病毒株存在导致金刚烷胺耐药的M2蛋白V27A突变。全部14个病毒株PB1-F2蛋白发生I18T突变。结论 M2蛋白耐药突变的出现和PB1-F2蛋白氨基酸突变提示分离获得的H3N2流感病毒株在进化过程中曾经结合H1N1亚型流感病毒基因片段。HA1蛋白抗原决定簇突变的增加可能引发患者更强的免疫反应。
[Abstract]:Objective to analyze the whole genome sequence of influenza virus strain H3N2 and its correlation with clinical features. Methods the nasal swabs were detected from December 2014 to March 2015 in patients with respiratory tract infection. Ben. The H3N2 subtype influenza virus strain was amplified by PCR and sequenced directly. The nucleic acid sequence and amino acid variation were analyzed. The results were compared with the reference strain recommended by WHO for influenza vaccine strain 2014-2015. The HA1 protein determinants of 14 virus strains from different patients were mutated to different degrees. The host had higher body temperature and more severe clinical manifestation. All 14 strains had HA1 protein E190D amino acid mutation and 6 virus strains had na eggs. There was a new glycosylation site in white. One virus strain had a M2 protein V27A mutation that led to amantadine resistance. All 14 virus strains had I18T mutation in PB1-F2 protein. The emergence of M2 protein resistance mutation and amino acid mutation of PB1-F2 protein suggest that the isolated H3N2 influenza virus strain has been linked to the H1N1 subtype influenza virus gene fragment. HA1 egg during the evolution of H3N2 influenza virus strain. An increase in mutations in the determinant of white antigen may trigger a stronger immune response in patients.
【作者单位】：上海交通大学医学院附属瑞金医院感染病与呼吸病研究所临床病毒研究室;上海交通大学医学院附属瑞金医院呼吸科;上海交通大学医学院附属瑞金医院北院;
【基金】：上海申康前沿技术联合攻关项目~~
【分类号】：R511.7
【正文快照】： com bined w ith gene fragm ents of influenza virus H1N1 during the course of evolution.T he increase of variationsof the epitope of H A1 protein m ay cause stronger im m une response of patients.流感病毒(influenza virus)自1902年首次分离之后一直是引起人类

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