两例输入性登革病毒合并基孔肯雅病毒感染实验室检测
发布时间:2018-01-16 12:28
本文关键词:两例输入性登革病毒合并基孔肯雅病毒感染实验室检测 出处:《病毒学报》2017年02期 论文类型:期刊论文
【摘要】:对不明原因发热病人开展登革病毒和基孔肯雅病毒合并感染的检测,明确合并感染的可能性,为防御这两种虫媒传染病的传播提供依据。通过流行病史调查,临床症状和实验室检测对两名从泰国普吉岛旅行归来的发热病人进行了登革病毒和基孔肯雅病毒感染的诊断。两名患者在泰国旅行停留10天,在此期间两人均有蚊虫叮咬史。回国3天后两患者相继出现高热症状(39℃以上),且伴有皮疹,肌肉酸痛和乏力。实验室血常规检测发现轻度血小板降低伴淋巴细胞减少,登革病毒IgG/IgM快速诊断试剂盒检测发现一名患者登革病毒IgM阳性。实时荧光RT-PCR检测证实两患者血液中登革病毒和基孔肯雅病毒核酸均阳性,同时用RT-PCR方法扩增获得了登革病毒C-prM蛋白部分基因,经测序和同源性分析,证实感染登革病毒属于Ⅰ型登革热病毒。这是我国首次出现的输入性登革病毒合并基孔肯雅病毒感染病例,本研究建议对流行病史和临床症状满足的病例要同时进行两种病毒的实验室检测。
[Abstract]:To detect the co-infection of dengue virus and Kikungunya virus in patients with fever of unknown origin, to determine the possibility of co-infection, to provide the basis for the prevention of the spread of these two kinds of insect-borne infectious diseases, and to investigate the epidemic history. Clinical symptoms and laboratory tests diagnosed two febrile patients returning from a trip to Phuket, Thailand, with dengue virus and Kikungunya virus infection. The two patients spent 10 days traveling in Thailand. During this period, both of them had a history of mosquito bites. After 3 days of return to China, the two patients developed hyperthermia symptoms above 39 鈩,
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