基因失活菌株产生的2个雷莫拉宁类似物及其活性测定

发布时间：2018-01-19 23:57

本文关键词： 雷莫拉宁生物合成分离纯化抑菌活性　出处：《中国抗生素杂志》2015年09期 　论文类型：期刊论文

【摘要】：目的以基因工程菌A.CJS1001与SIPI-GE.2006 dorf20为研究对象,获得雷莫拉宁类似物同时测定其生物活性。方法利用产生菌的自然选育,雷莫拉宁类似物的分离纯化,HPLC检测产量变化及MIC检测。结果获得高产菌株A.CJS1011与SIPI-GE.2016 dorf20,得到无糖雷莫拉宁的量为120mg;无氯雷莫拉宁的量为203mg,其主要成分A2的HPLC显示纯度均大于90%。生物活性检测显示:无糖雷莫拉宁A2对耐甲氧西林金黄色葡萄球菌株ATCC43300的抑菌浓度为2μg/m L,对耐万古霉素肠球菌ATCC51299的抑菌浓度为1μg/m L;无氯雷莫拉宁A2对耐甲氧西林金黄色葡萄球菌株ATCC43300的抑菌浓度为2μg/m L,对耐万古霉素肠球菌株ATCC51299抑菌浓度为2μg/m L。结论以生物合成的方法分离纯化获得雷莫拉宁的类似物为进一步研究雷莫拉宁奠定了基础。
[Abstract]:Objective to study the genetic engineering bacteria A.CJS1001 and SIPI-GE.2006 dorf20. The bioactivity of remolanine analogue was determined simultaneously. Methods the natural breeding of the producing strain and the separation and purification of the Ramolanine analogue were carried out. Results the high yield strains A.CJS1011 and SIPI-GE.2016 dorf20 were obtained. The yield of amoranine was 120 mg; The dosage of chloramoranine was 203mg. The HPLC purity of A2 was higher than 90. The bioactivity test showed that:. The inhibitory concentration of amoranine A2 against methicillin-resistant Staphylococcus aureus strain ATCC43300 was 2 渭 g / mL. The inhibitory concentration of vancomycin resistant Enterococcus ATCC51299 was 1 渭 g / mL; The inhibitory concentration of chlorine free remolanine A2 against methicillin-resistant Staphylococcus aureus strain ATCC43300 was 2 渭 g / mL. The inhibitory concentration of ATCC51299 against vancomycin resistant Enterococcus strains was 2 渭 g / m. Conclusion the analogue of remolanine was isolated and purified by biosynthesis, which laid a foundation for further study of remolanine.
【作者单位】：中国医药工业研究总院上海医药工业研究院创新药物与制药工艺国家重点实验室;
【基金】：国家自然科学基金面上项目(No.81373310,No.81273413) “重大新药创制”国家重大项目专项资助(No.2012ZX09304004)
【分类号】：R515
【正文快照】： 雷莫拉宁(r a m o p l a n i n)是在游动放线菌Actinoplanes sp.ATCC 33076的发酵液中发现的[1],它包含A1、A2、A3 3个组分,其中A2是主要成分。雷莫拉宁的骨架结构是由17个氨基酸残基组成的肽链,通过3-氯-4-羟基苯甘氨酸残基和β-羟基天门冬酰胺残基之间的内酯键相连,形成49元

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