三苯双脒治疗旋毛虫感染小鼠血清IL-4和TGF-β1的变化

发布时间：2018-01-20 11:16

本文关键词： 旋毛虫三苯双脒白细胞介素- 转化生长因子-β　出处：《中国病原生物学杂志》2015年02期 　论文类型：期刊论文

【摘要】：目的观察旋毛虫感染小鼠经三苯双脒治疗后血清中细胞因子IL-4和TGF-β1水平变化。方法将120只昆明小鼠随机分成,感染对照组、治疗组和正常对照组,每组40只。感染对照组和治疗组小鼠经灌胃感染云南株旋毛虫肌幼虫200条/只。治疗组小鼠于感染后第7d经灌胃给予三苯双脒治疗,每只每天300mg体重,连续7d。分别在感染后7、14、21、28和35d对各组小鼠采血,分离血清,采用ELISA检测IL-4和TGF-β1水平。结果 ELISA检测显示,感染对照组和治疗组IL-4、TGF-β1水平在感染旋毛虫后开始升高,在感染后14d达到峰值,分别为(40.17±1.01)pg/ml、(100.23±1.31)pg/ml、和(30.21±1.25)pg/ml、(80.51±1.37)pg/ml,随后逐渐下降。在感染后5个时间点,感染对照组小鼠血清IL-4和TGF-β1水平与正常对照组比较差异均有统计学意义(P0.01);治疗组IL-4和TGF-β1水平在感染后5个时间点与感染对照组(P0.01),与正常对照组比较差异均有统计学意义。结论三苯双脒治疗旋毛虫感染小鼠后血清IL-4和TGF-β1水平随感染时间先升高后降低。
[Abstract]:Objective to observe the changes of serum levels of cytokines IL-4 and TGF- 尾 1 in mice infected with Trichinella spiralis. Methods 120 Kunming mice were randomly divided into two groups. Treatment group and normal control group. There were 40 mice in each group. The mice in the infection control group and the treatment group were infected with 200 muscle larvae of Trichinella yunnanensis by gavage. The mice in the treatment group were treated with tribendimidine by gavage on the 7th day after infection. The blood samples were collected from each mouse on day 28 and 35 after infection, and the serum samples were collected from each group for 7 days. Results the levels of IL-4 and TGF- 尾 1 were detected by ELISA. Results the levels of IL-4 and TGF- 尾 1 in infected control group and treatment group began to increase after infection with Trichinella spiralis. On the 14th day after infection, the peak value was 40.17 卤1.01g / ml, 100.23 卤1.31g / ml, respectively. And 30.21 卤1.25 PG / ml, 80.51 卤1.37 g / ml, then gradually decreased. At 5 time points after infection. The serum levels of IL-4 and TGF- 尾 1 in the infected control group were significantly higher than those in the normal control group (P 0.01). The levels of IL-4 and TGF- 尾 1 in the treatment group were compared with those in the control group at 5 time points after infection (P 0.01). Conclusion the serum levels of IL-4 and TGF- 尾 1 in mice infected with Trichinella spiralis increased first and then decreased with the time of infection.
【作者单位】：承德医学院病原生物学教研室;
【分类号】：R532.14
【正文快照】： *旋毛形线虫(Trichinella spiralis)简称旋毛虫,可以感染人及猪、鼠、熊等150多种哺乳动物,引起旋毛虫病。该病呈世界性分布[1-2],人主要因食入生或半生含旋毛虫幼虫囊包的肉类而感染旋毛虫病,重症患者可致死亡[3]。三苯双脒(tribendimidine,TBD)作为一种新型抗蠕虫药,近年来

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