系统性红斑狼疮合并真菌感染患者NK细胞功能的变化

发布时间：2018-01-24 03:57

本文关键词： 系统性红斑狼疮 NK细胞真菌感染　出处：《中南大学》2014年硕士论文　论文类型：学位论文

【摘要】：研究背景和研究目的：系统性红斑狼疮是一种以高滴度的自身抗体、循环免疫复合物升高以及补体下降为主要特点,多种临床表现为主要特征,多因素参与的自身免疫性疾病,其发病机制未明。而NK细胞是固有免疫的重要组成部分,是抵抗外来病原体侵犯的第一道防线。本实验的目的主要是分析NK细胞在SLE患者中免疫防御功能的改变。研究方法：收集2013年8月至2014年4月在中南大学湘雅医院确诊的SLE. SLE合并肺部真菌感染、肺部真菌感染患者及健康体检者的外周血。将实验组分为四组,即SLE组(n=20)、SLE合并肺部真菌感染组(n=14)、肺部真菌感染组(n=14)和健康对照组(n=20),用流式细胞术分别检测各组患者和健康人外周血中CD3-CD56+NK细胞数、NK表面受体NKp44和TLR2、胞内颗粒酶B的表达。实验结果：在SLE与SLE合并肺部真菌感染患者外周血中,NK细胞数目较其他两组明显下降(P0.05),而两组间比较无显著差异(P0.05),肺部真菌感染组较正常组升高,差异无显著性(P0.05)；颗粒酶B在SLE组外周血中的表达较健康对照组及肺部真菌感染组明显减少(P0.05),而在SLE合并肺部真菌感染组中,颗粒酶B表达较健康对照组明显增高(P0.05),且较SLE组和肺部真菌感染组也明显增高(P0.05),肺部真菌感染组较健康对照组无明显差异；NKp44在SLE合并肺部真菌感染患者中NK细胞的表达较其他三组明显升高(P0.05); TLR2在SLE组及SLE合并肺部真菌感染组中的表达较健康对照组及肺部真菌感染组明显升高(P0.05),SLE合并肺部真菌感染组较SLE组表达减少,而肺部真菌感染组较健康对照组明显增加(P0.05)。结论：在系统性红斑狼疮患者体内,NK细胞及颗粒酶B的比例较正常组明显减少,其免疫防御功能降低；TLR2的表达在SLE患者合并肺部真菌感染组中较SLE组降低,而在肺部真菌感染组中的表达较正常组增高；系统性红斑狼疮患者合并真菌感染时,NKp44表达较健康组及SLE组上调,并伴有TLR2和颗粒酶B的明显升高,NK细胞功能被激活。
[Abstract]:Background and objective: systemic lupus erythematosus (SLE) is characterized by high titer autoantibodies, elevated circulating immune complex and decreasing complement. The pathogenesis of multifactorial autoimmune diseases is not clear, and NK cells are an important part of innate immunity. It is the first line of defense against the invasion of foreign pathogens. The purpose of this study was to analyze the changes of immune defense function of NK cells in patients with SLE. Methods: collected from August 2013 to April 2014 in Xiangya Hospital of Central South University diagnosed SLE. SLE with pulmonary fungal infection. Peripheral blood of patients with pulmonary fungal infection and healthy persons. The experimental group was divided into four groups: SLE group with pulmonary fungal infection. The number of CD3-CD56 NK cells in peripheral blood of patients with pulmonary fungal infection and control group were detected by flow cytometry. Expression of NK receptor NKp44 and TLR 2 and intracellular granzyme B expression. Results: the number of NK cells in peripheral blood of SLE and SLE patients with pulmonary fungal infection was significantly lower than that of the other two groups (P 0.05). There was no significant difference between the two groups (P 0.05), but the pulmonary fungal infection group was higher than the normal group (P 0.05). The expression of granzyme B in peripheral blood of SLE group was significantly lower than that of healthy control group and pulmonary fungal infection group, but in SLE complicated with pulmonary fungal infection group. The expression of granzyme B was significantly higher than that of the healthy control group (P 0.05), and the expression of granzyme B was significantly higher than that of the SLE group and the pulmonary fungal infection group. There was no significant difference between the pulmonary fungal infection group and the healthy control group. The expression of NKp44 in SLE patients with pulmonary fungal infection was significantly higher than that in the other three groups. The expression of TLR2 in SLE group and SLE with pulmonary fungal infection group was significantly higher than that in healthy control group and pulmonary fungal infection group (P0.05). Compared with SLE group, the expression of P0. 05 in SLE with pulmonary fungal infection group was significantly higher than that in healthy control group. Conclusion: the percentage of NK cells and granzyme B in patients with systemic lupus erythematosus is significantly lower than that in the normal group, and its immune defense function is decreased. The expression of TLR2 in SLE patients with pulmonary fungal infection was lower than that in SLE group, but the expression in pulmonary fungal infection group was higher than that in normal group. The expression of NKp44 in patients with systemic lupus erythematosus complicated with fungal infection was higher than that in healthy group and SLE group, and the function of NK cells was activated with the increase of TLR2 and granzyme B.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R593.241;R519

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