福建省EV71流行与遗传变异特点分析

发布时间：2018-01-28 11:31

本文关键词： 肠道病毒型流行病学遗传特征疫苗预防　出处：《中国人兽共患病学报》2017年02期 　论文类型：期刊论文

【摘要】：目的分析2010-2015年福建省肠道病毒71型(EV71)流行概况及病毒VP1基因特征,为今后EV71疫苗在福建省的应用提供参考。方法基于手足口病监测系统,对福建省2010-2015年间EV71流行情况进行描述性流行病学分析。随机选取72株病毒分离株,扩增病毒VP1区并与疫苗株作序列进化分析。比较两者核苷酸及氨基酸序列差异性,分析分离株在VP1蛋白4个免疫表位的氨基酸变异情况。结果 2010-2015年,EV71总体感染率呈下降趋势,在每年的5-7月均出现一次流行高峰;发病地区主要集中在厦门、福州、南平、泉州;主要感染1-3岁年龄段儿童,男性比例高于女性,职业分布以散居儿童为主。重症病例中EV71比例高于在总病例中的比例(χ2=732.064 5,P0.000 1)。福建地区流行的EV71病毒均属于C4a亚型,与疫苗株的核苷酸差异性随时间距离增加而逐渐增大,氨基酸差异性未有明显变化;在4个免疫表位上的氨基酸变异未发现年度上的规律和同HMFD中EV71构成分布相符的变化。结论 2010年至2015年,福建地区流行的EV71具有明显的时间、地点、人群分布特征,72株病毒株均属于C4a基因亚型,与疫苗株相比,VP1区未出现显著的抗原漂移及变异。应继续保持对EV71流行病学监测和遗传特征的分析,为今后疫苗的应用提供更为详尽的资料。
[Abstract]:Objective to analyze the prevalence of enterovirus 71 (EV71) and the characteristics of VP1 gene in Fujian Province from 2010 to 2015. To provide a reference for the future application of EV71 vaccine in Fujian Province. Methods based on the hand-foot-mouth disease surveillance system. The epidemic situation of EV71 in Fujian Province from 2010 to 2015 was analyzed by descriptive epidemiology. 72 strains of virus were randomly selected. The VP1 region of the virus was amplified and the nucleotide and amino acid sequence difference was compared with that of the vaccine strain. The amino acid variation of the four immune epitopes of VP1 protein was analyzed. Results the overall infection rate of EV71 decreased from 2010 to 2015. An epidemic peak appeared in May-July of each year. The incidence areas were mainly in Xiamen, Fuzhou, Nanping, Quanzhou; The proportion of males was higher than that of females, and the occupational distribution was mainly scattered children. The proportion of EV71 in severe cases was higher than that in total cases (蠂 2 732 064 5). The nucleotide difference between P0. 000 1 and vaccine strain increased with time, but the difference of amino acid did not change significantly in Fujian province. All of the EV71 viruses in Fujian were C4a subtype, and the nucleotide difference increased gradually with the increase of time distance between the EV71 virus and the vaccine strain. The amino acid variation in the four immune epitopes was not found to be consistent with the distribution of EV71 composition in HMFD. Conclusion from 2010 to 2015. The prevalence of EV71 in Fujian has obvious time, location and population distribution characteristics. 72 strains belong to C4a gene subtype, compared with vaccine strain. There was no significant antigen drift and variation in the VP1 region. The epidemiological monitoring and genetic analysis of EV71 should be maintained so as to provide more detailed information for the application of vaccines in the future.
【作者单位】：福建省人兽共患病研究重点实验室福建省疾病控制预防中心;
【基金】：国家级科技发展研究计划(2011AA02A114)资助~~
【分类号】：R512.5;R181.3
【正文快照】： 员,主要感染儿童。上世纪70-80年代,EV71主要在欧美、澳大利亚等地流行,除局部暴发外,多为低水平流行[1]。进入90年代亚洲成为EV71的主要流行地区,包括新加坡,马来西亚,台湾地区[2-4]。EV71是儿童手足口病的主要病原体,是引起手足口病(Hand,Foot and Mouth Disease,HMFD)重症

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