脑型血吸虫病的磁共振早期诊断——家兔急性脑型血吸虫病模型建立

发布时间：2018-01-29 14:38

  本文关键词： 日本血吸虫 脑型血吸虫病 磁共振成像 动物模型 虫卵肉芽肿　出处：《中国血吸虫病防治杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的建立家兔急性脑型血吸虫病模型,探讨急性血吸虫脑病早期磁共振表现。方法家兔分3组,每组10只;实验组通过钻颅法注射日本血吸虫虫卵悬浮液1 ml(0.9 mg);阴性对照组用相同方法经颅注射生理盐水1 ml;空白对照组不做任何处理。前两组术后使用抗生素预防感染。观察3组动物的临床表现,并于术后30 d行头颅磁共振检查;随后取脑组织制作病理切片,观察脑组织病理改变。结果实验组家兔术后均出现食欲不振、精神异常,偏瘫及体重下降等症状;阴性对照组家兔术后3 d内均出现食欲下降,1周后消失,无异常精神症状;空白对照组无不良反应。实验组10只家兔磁共振表现均发现异常,表现为T1WI增强,出现片状、结节状强化,脑水肿,脑室扩张,注射针道异常强化,SWI脑内强化结节内异常点低信号及手术侧脑半球内片状低信号。阴性对照组2只磁共振表现为针道异常强化,1只脑室轻度扩张。空白对照组磁共振表现无异常。实验组10只脑组织病理切片均发现异常,其中6只为血吸虫虫卵肉芽肿结节、非特异性肉芽肿结节以及血管周围炎并存;阴性对照组未发现肉芽肿结节,2只出现血管周围炎;空白对照组脑组织切片正常。结论经颅注射血吸虫虫卵可成功建立家兔急性血吸虫脑病模型;通过观察磁共振图像总结早期血吸虫脑病表现,有助于提高早期诊断的正确率。
[Abstract]:Objective to establish an acute cerebral schistosomiasis model in rabbits and to investigate the early MRI manifestations of acute schistosomiasis. Methods Rabbits were divided into 3 groups with 10 rats in each group. In the experimental group, Schistosoma japonicum egg suspension was injected into the egg suspension of Schistosoma japonicum 1 ml(0.9 by drilling the skull. The negative control group was injected with 1 ml of normal saline by the same method. No treatment was given in the blank control group. The first two groups were treated with antibiotics to prevent infection after operation. The clinical manifestations of the three groups were observed and cranial magnetic resonance imaging was performed 30 days after operation. The pathological changes of brain tissue were observed. Results all the rabbits in the experimental group had symptoms of loss of appetite, mental abnormality, hemiplegia and weight loss after operation. In the negative control group, the appetite decreased within 3 days after operation and disappeared after 1 week, and no abnormal mental symptoms were found. There were no adverse reactions in the blank control group. All the 10 rabbits in the experimental group showed abnormal MRI findings, such as T 1WI enhancement, flake enhancement, nodular enhancement, cerebral edema, ventricular dilatation and abnormal enhancement of the injection needle. In SWI, the abnormal points in the nodules were hypointensity and the lamellar hypointensity in the operative hemispheres. In the negative control group, 2 cases showed abnormal enhancement of the needle tract. One brain ventricle was slightly dilated. There was no abnormal MRI in the blank control group. In the experimental group, 10 brain tissue sections showed abnormal findings, 6 of which were egg granuloma nodules of Schistosoma japonicum. Non-specific granulomatous nodules and perivascular inflammation coexist. No granulomatous nodules were found in the negative control group. Conclusion the rabbit model of acute schistosomiasis can be successfully established by transcranial injection of Schistosoma japonicum eggs. It is helpful to improve the accuracy of early diagnosis by observing magnetic resonance imaging to summarize the manifestations of early schistosomiasis encephalopathy.
【作者单位】： 江南大学附属医院;江南大学公共卫生学院;武警浙江省总队医院放射科;江苏省血吸虫病防治研究所;浙江中医药大学动物实验研究中心;江南大学医学院;
【基金】：江南大学公共卫生研究中心立项项目(1286010242150640)
【分类号】：R-332;R532.21
【正文快照】： 江南大学公共卫生研究中心立项项目(1286010242150640)1江南大学附属医院(无锡214000);2江南大学公共卫生学院;3武警浙江省总队医院放射科;4江苏省血吸虫病防治研究所;5浙江中医药大学动物实验研究中心;6江南大学医学院葛宇曦,女,研究生,主治医师。研究方向:磁共振诊断*通信作，

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