慢性HBV感染者外周血Treg水平与HBV-DNA载量的相关性研究

发布时间：2018-01-31 18:23

本文关键词： 调节性T细胞 HBV-DNA HBV感染　出处：《中国全科医学》2015年28期 　论文类型：期刊论文

【摘要】：目的探讨慢性HBV感染者外周血CD4+CD25+调节性T细胞(Treg)水平与HBV-DNA载量的相关性。方法选取2013年1月—2014年12月石河子大学医学院第一附属医院感染科收治的住院和门诊慢性HBV感染者100例,其中轻中度慢性乙型肝炎(CHBLM)组43例、乙型肝炎肝硬化(LC)组27例、慢性重型乙型肝炎(CSHB)组30例;选取同期该院健康体检者36例为健康对照(HC)组。采用实时荧光定量聚合酶链反应法(FQ-PCR)检测HBV-DNA载量,采用流式细胞仪检测Treg水平。外周血Treg水平与HBV-DNA载量的相关性采用Pearson相关分析。结果 CHBLM、LC、CSHB组的HBV-DNA载量比较,差异有统计学意义(F=4.564,P0.05);两两比较显示,CHBLM组与LC组间差异有统计学意义(P0.05),其他组间差异无统计学意义(P0.05)。CHBLM、LC、CSHB、HC组的Treg水平比较,差异有统计学意义(F=25.218,P0.05);组间两两比较显示,组间差异均有统计学意义(P0.05)。CHBLM、LC、CSHB组外周血Treg水平与HBV-DNA载量均呈正相关(r值分别为0.458、0.697、0.544,P0.05)。结论慢性HBV感染者Treg水平增加,Treg水平与HBV-DNA载量呈正相关。提示Treg在慢性HBV感染中担负着重要的免疫调节作用,可能抑制HBV感染者特异性细胞免疫反应,与HBV感染的慢性化及肝病的临床发生、发展有关。
[Abstract]:Objective to investigate the regulatory T cell count of peripheral blood CD4 CD25 in patients with chronic HBV infection. Methods from January 2013 to December 2014, the patients with chronic HBV infection were selected from the first affiliated Hospital infection Department of Shihezi University Medical College. 100 cases. There were 43 cases in CHBLM group, 27 cases in LC group and 30 cases in CSHBs group. In the same period, 36 healthy persons were selected as the healthy control group. The HBV-DNA load was detected by real-time fluorescence quantitative polymerase chain reaction (FQ-PCR). The level of Treg was detected by flow cytometry and the correlation between Treg level and HBV-DNA load in peripheral blood was analyzed by Pearson correlation analysis. The difference of HBV-DNA load in CSHB group was statistically significant (P 0.05). Pairwise comparison showed that the difference between CHBLM group and LC group was statistically significant (P 0.05), but there was no significant difference between other groups (P 0.05). The difference of Treg level in HC group was statistically significant (P 0.05). The comparison between two groups showed that there was significant difference between the two groups (P 0.05N. CHBLMN LC). There was a positive correlation between the level of Treg and the load of HBV-DNA in CSHB group. Conclusion the level of Treg in patients with chronic HBV infection is increased. The level of Treg was positively correlated with the load of HBV-DNA, suggesting that Treg plays an important role in the immunomodulation of chronic HBV infection and may inhibit the specific cellular immune response of HBV infected patients. It is related to the chronic infection of HBV and the clinical occurrence and development of liver disease.
【作者单位】：石河子大学医学院第一附属医院感染科;新疆兵团第九师医院感染科;
【分类号】：R512.62
【正文快照】： HBV感染后可能发展为慢性HBV携带、慢性肝炎、肝硬化、肝癌,其病情容易慢性化,发病机制复杂且至今尚未完全阐明,目前认为除与HBV的直接作用有关外,也与宿主免疫应答调控的紊乱、体内缺乏特异性抗病毒免疫关系密切,宿主的免疫应答同时导致了肝细胞的损伤和病毒的清除两个方面,

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