MicroRNA-146a基因多态性与慢性乙型肝炎患者抗病毒疗效的相关性

发布时间：2018-02-02 22:14

本文关键词： RNA 基因多态性肝炎病毒等位基因　出处：《中国现代医学杂志》2015年35期 　论文类型：期刊论文

【摘要】：目的探讨micro RNA-146a(mi R-146a)基因单核苷酸多态性(SNP)和慢性乙型病毒性肝炎(CHB)患者干扰素-α(IFN-α)治疗应答反应的相关性。方法选择接受IFN-α抗病毒治疗的慢性乙型肝炎患者133例,其中应答组79例,无应答组54例。采集两组静脉血并提取DNA,用聚合酶链式反应-限制性内切酶长度多态性(PCR-RFLP)方法对mi R-146a行SNP分型。结果 mi R-146a CT基因型与TT基因型比较,差异有统计学意义(P=0.009);CC基因型与TT基因型比较,差异有统计学意义(P=0.029)。T等位基因与C等位基因频率比较差异有统计学意义(P=0.020)。结论 Mi R-146a基因多态性与慢性乙型肝炎患者抗病毒疗效相关,T等位基因或TT基因型与IFN治疗应答相关,而C等位基因或CC基因型与干扰素治疗应答无相关。
[Abstract]:Objective to investigate interferon- 伪 (IFN- 伪) in patients with micro RNA-146a(mi R-146a) and chronic hepatitis B (CHB) patients with single nucleotide polymorphisms (SNPs). Methods 133 patients with chronic hepatitis B received IFN- 伪 antiviral therapy. There were 79 cases in the response group and 54 cases in the non-response group. Venous blood was collected and DNA was extracted from the two groups. PCR-RFLP using polymerase chain reaction-restriction endonuclease length polymorphism. Methods SNP typing was performed on mi R-146a. Results the CT genotypes of mi R-146a were compared with those of TT genotypes. The difference was statistically significant (P < 0. 009). CC genotype was compared with TT genotype. There was a significant difference in the frequency of allele C and T allele (P 0. 029) and the frequency of C allele was significantly different (P < 0. 020). Conclusion Mi R-146a gene polymorphism is associated with antiviral efficacy in patients with chronic hepatitis B. T allele or TT genotype was associated with IFN response, while C allele or CC genotype had no correlation with interferon response.
【作者单位】：广东省汕头市中心医院;
【分类号】：R512.62
【正文快照】： 乙型肝炎病毒(hepatitis B virus,HBV)是一种导致急、慢性乙型肝炎的病原体,世界上约有4亿慢性HBV感染者,其中75%为亚洲人。急性感染可导致严重致命的爆发性肝炎,慢性感染可导致肝硬化甚至肝细胞癌,所以在许多国家,这是一非常严重的问题[1]。干扰素-α(Interferon-α,IFN-α)

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