新建HIV-1新发感染快速检测方法与ELISA法的比较研究

发布时间：2018-02-02 23:09

本文关键词： 艾滋病病毒新发感染金标银染免疫渗滤快速检测方法限制性抗原亲和力方法 BED捕获酶联免疫法抗病毒治疗　出处：《中国艾滋病性病》2017年06期 　论文类型：期刊论文

【摘要】：目的初步评价艾滋病病毒Ⅰ型(HIV-1)新发感染快速检测方法,在横断面现场调查人群样本中的应用能力,并分析抗病毒治疗对该方法的影响。方法应用该研究前期构建的金标银染免疫渗滤快速检测方法(简称快速检测法),检测2014年江西省国家级哨点新报告的HIV-1感染者和抗病毒治疗者的样本。将新发感染判定的结果与限制性抗原亲和力方法(LAg Avidity EIA,简称LAg法)、BED捕获酶免疫法(BED CEIA,简称BED法)进行一致性和相关性比较,进一步分析三种方法检测抗病毒治疗者样本的差异。结果纳入研究的新发感染者样本共122份,快速检测法与LAg法、BED法判定新发感染结果的一致率分别为94.26%和88.52%。快速检测法检测探针灰度值与LAg法和BED法的ODn值具有较高的相关性。R2分别为0.947 5与0.923 4。经配对卡方检验分析,快速检测法判定新发感染的比例显著低于BED法(P0.001),而与LAg法差异无统计学意义(P=0.257)。纳入研究的抗病毒治疗者的样本共117份,快速检测法对其误判为近期的比率为16.24%(19/117),而BED方法和LAg方法的误判比率分别为16.24%(19/117)、14.53%(17/117)。另外,快速检测法对所有样本常规HIV抗体检测结果均正确。结论金标银染免疫渗滤快速检测方法检测HIV-1新发感染具有良好的准确性和实际应用性。但抗病毒治疗对快速检测法影响较大,应在横断面人群调查时删除抗病毒治疗样本。
[Abstract]:Objective to evaluate the ability of rapid detection of HIV type I (HIV-1) infection in a cross-sectional survey of population samples. The effect of antiviral therapy on this method was analyzed. Methods the rapid detection method of gold labeled silver staining immunofiltration was used in the early stage of the study. In 2014, the samples of newly reported HIV-1 infected persons and antiviral therapists in Jiangxi Province were detected. The results of the new infection determination were compared with the restrictive antigen affinity method (. LAg Avidity EIA. The consistency and correlation between the two methods were compared by LAg method and BED method. Further analysis of the three methods to detect the difference of antiviral treatment samples. Results A total of 122 newly infected individuals were included in the study, rapid detection method and LAg method. The results of BED were 94.26% and 88.52, respectively. The gray value of probe detected by rapid detection method had a high correlation with the ODn value of LAg method and BED method. 2 = 0.947 respectively. 5 and 0.923 4. Analysis by paired chi-square test. The proportion of new infections detected by rapid detection method was significantly lower than that by BED method (P 0.001). However, there was no significant difference between the two methods (P < 0. 257). A total of 117 samples of antiviral therapy were included in the study. The recent rate of miscalculation by the rapid detection method was 16.24 to 19 / 117, while that of the BED and LAg methods was 16.2419 / 117, respectively. 14.53 / 17 / 117. Conclusion the rapid detection method of gold standard silver staining immunofiltration has good accuracy and practical application in detecting new HIV-1 infection, but it is resistant to disease. Toxic therapy has great influence on rapid detection. Antiviral treatment samples should be removed from cross-sectional population surveys.
【作者单位】：中国疾病预防控制中心性病艾滋病预防控制中心参比室;河北医科大学;江西省疾病预防控制中心;首都医科大学附属北京佑安医院;北京金豪制药股份有限公司;
【基金】：北京市科委课题(D161100000416001)~~
【分类号】：R512.91
【正文快照】： Supported by Beijing Science and Technology Project(D161100000416001)艾滋病病毒(HIV)新发感染率是评价艾滋病(AIDS)流行趋势和防治措施的重要公共卫生指标[1-2],目前普遍采用简便经济的实验室方法从横断面样本中检测新发感染来进行估算。随着实验技术的进步,现在已有20

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