上海市静安区2013年新报告感染者的HIV-1分子流行病学研究

发布时间：2018-02-03 14:01

本文关键词： 艾滋病病毒-型基因亚型重组体流行病学分子　出处：《中国艾滋病性病》2016年05期 　论文类型：期刊论文

【摘要】：目的了解上海市静安区2013年新发现的、未经抗病毒治疗的艾滋病病毒1型(HIV-1)感染者的亚型分布特征,为该区制定艾滋病防治策略提供科学依据。方法选取2013年新发现的HIV-l感染者血浆标本144份,运用反转录-聚合酶链反应(RT-PCR)扩增,进行DNA测序及系统进化树分析,并结合流行病学资料进行分析。结果⑴获得104份pol区基因片段,其中亚型分布显示,CRF01_AE亚型所占比例最大,为73.1%(76/104),其次为CRF07_BC为19.2%(20/104);B亚型为4.8%(5/104);CRF08_BC为1.9%(2/104);并发现新的重组体CRF 59_01B亚型为1.0%(1/104)。⑵HIV-1基因亚型与年龄分布(χ~2=17.72,P0.05)、传播途径有统计学关联(χ~2=20.11,P0.05),和CD4+T淋巴细胞分组(χ2=12.83,P0.05)之间有统计学差异。⑶Logistic回归分析显示,50岁以上及基因亚型是HIV进展为艾滋病的主要影响因素。结论男男同性性传播是静安区HIV-1感染的主要传播途径,HIV-1感染者中病毒基因亚型具有多样化,应加强对感染者的基因亚型监测。
[Abstract]:Objective to investigate the subtype distribution of HIV type 1 (HIV 1) infected persons without antiviral therapy in 2013 in Jing'an District of Shanghai. Methods 144 HIV-l infected plasma samples were collected in 2013. Reverse transcription-polymerase chain reaction (RT-PCR) amplification was used for DNA sequencing and phylogenetic tree analysis. Results 1 104 pol gene fragments were obtained, among which the distribution of subtypes showed that CRF01AE subtype accounted for the largest proportion. It was 73.1 / 104, followed by CRF07_BC 19.2 / 104m; B subtype was 4.8 / 104; CRF08_BC was 1.9 / 104; It was also found that the subtype of the new recombinant CRF 5901B was 1.0 / 104 / 104. HIV-1 gene subtype and age distribution (蠂 ~ (2 / 2) 17.72 ~ (2) P 0.05). There was a statistical correlation between the transmission route (蠂 2 + 20. 11 P 0. 05) and the CD4 T lymphocyte group (蠂 2 + 12. 83). P 0.05). 3 Logistic regression analysis showed that there was a statistical difference between the two groups. Age over 50 and gene subtype are the main influencing factors of HIV progression. Conclusion MSM is the main route of transmission of HIV-1 infection in Jing'an district. The subtypes of virus genes in HIV-1 infected patients are diverse, so we should strengthen the monitoring of the subtypes of virus genes.
【作者单位】：上海市静安区疾病预防控制中心;上海市疾病预防控制中心;
【基金】：国家“十二五”科技重大专项(2012ZX10001-002-002-012) 上海市静安区卫生计生系统第三批专业技术骨干项目(JWRC2014G14)~~
【分类号】：R512.91
【正文快照】： Supported by the National"Twelfth Five-year"key SdT Special Projects(2012ZX10001-002-002-012)and"Ten Hundred Thousand"training project from Jingan District Health Board(JWRC2014G14)截至2013年底全国报告存活艾滋病病毒1型(Human immunodeficiency virus-1,HIV-

【相似文献】