新型甲型H1N1流感重症病例肝脏病理学变化分析

发布时间：2018-02-09 02:01

本文关键词： 新型甲型HN流感肝脏病理特征 CD 转化生长因子β(TGF-β_)　出处：《首都医科大学学报》2015年03期 　论文类型：期刊论文

【摘要】：目的从组织、细胞、蛋白水平,观察新型甲型H1N1流感病毒感染后肝脏的病理学变化特征,分析血凝素(hemagglutinin,HA)、核蛋白(nucleoprotein,NP)、CD68及转化生长因子β_1(transforming growth factorβ_1,TGF-β_1)在肝脏中的表达分布特点,以探讨重症死亡病例可能的发病机制。方法收集2009年北京甲型H1N1流感重症死亡系统解剖病例2例,床旁肝脏穿刺标本6例,通过常规病理学及免疫组织化学方法观察变化特征。结果肝脏存在不同程度充血及带状坏死,妊娠女性肝细胞伴有微泡型脂肪变。病毒抗原HA及NP在肝坏死区巨噬细胞中有少量表达;CD68主要表达在肝窦库普弗细胞及坏死区巨噬细胞;TGF-β_1主要表达在巨噬细胞,坏死区明显增多。结论新型甲型H1N1流感重症患者病理变化不仅局限于呼吸道,肝脏同样受累,其机制可能与CD68和TGF-β的作用相关。
[Abstract]:Objective to observe the pathological changes of liver after influenza A (H1N1) virus infection, and to analyze the expression and distribution of hemagglutinine HAA, nucleoprotein NPP CD68 and transforming growth factor 尾 1 growth factor 尾 1 (TGF- 尾 1) in the liver from tissue, cell and protein levels. Methods in order to explore the possible pathogenesis of severe death cases, 2 severe death cases and 6 bedside liver puncture specimens were collected in 2009. The changes were observed by routine pathological and immunohistochemical methods. Results there were various degrees of congestion and band necrosis in the liver. A small amount of viral antigen HA and NP were expressed in macrophages in the necrotic area of the liver, and CD68 was mainly expressed in the hepatic sinusoidal Kupffer cells and the necrotic macrophages, TGF- 尾 1 was mainly expressed in the macrophages. Conclusion the pathological changes of severe type A (H1N1) influenza patients are not only confined to the respiratory tract, but also involved in the liver, and the mechanism may be related to the role of CD68 and TGF- 尾.
【作者单位】：首都医科大学基础医学院病理系;首都医科大学附属北京佑安医院病理科;
【基金】：首都医学科技发展基金资助(2009-2091)~~
【分类号】：R511.7

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