贵阳地区无偿献血人群HCV筛查的结果分析

发布时间：2018-02-09 12:27

本文关键词： 肝炎丙型酶联免疫检测核酸检测确证实验　出处：《重庆医学》2017年17期 　论文类型：期刊论文

【摘要】：目的比较献血人群抗-HCV反应性、HCV核酸检测结果及HCV重组免疫印迹试验(RIBA)确证实验结果。方法对2013年10月至2015年3月采集的无偿献血者血液标本,采用2个不同厂家的国产酶联免疫吸附试验(ELISA)试剂检测和1个进口核酸检测试剂及配套检测系统对HCV进行筛查,对抗-HCV呈反应性或/和NAT检测阳性标本进行RIBA,将2种ELISA试剂检测反应性的结果与核酸检测结果、RIBA确证实验结果进行分析与比较。结果共检测133 959例无偿献血者标本,其中覆盖核酸检测结果的标本113 380例,抗-HCV检测呈反应性标本比例0.19%(252/133 959),NAT检测阳性共27例,阳性检出的比例0.02%(27/113 380);HCV反应性标本经RIBA确证实验确证阳性的比例19.8%(50/252)、阴性的比例54.8%(138/252)、不确定的比例25.4%(64/252);27例核酸检测阳性的标本均为ELISA检测双试剂反应性和确证实验阳性;2家ELISA试剂检测结果、RIBA确证实验结果和核酸检测结果差异有统计学意义(P0.05)。结论选用2次ELISA+1次核酸检测的检测策略更为安全;针对较高比例的假阳性标本,应建立献血者跟踪随访制度,最大限度地保留献血人群。
[Abstract]:Objective to compare the results of anti-HCV reactive HCV nucleic acid detection and HCV recombinant immunoblotting assay in blood donors. Methods Blood samples collected from unpaid blood donors from October 2013 to March 2015 were collected. HCV was screened by two different manufacturers of Elisa reagent, one imported nucleic acid detection reagent and a complete detection system. The reactivity and / or NAT positive specimens of anti-HCV were analyzed and compared with the results of nucleic acid assays confirmed by RIBA. A total of 133,959 volunteer blood donors were detected. Among them, 113 380 cases were covered with nucleic acid test results, 27 cases were positive for anti-HCV detection in the proportion of 0.19% and 252% / 133 959% Nat. The positive rate was 0.02%. The positive rate of 27 / 113 380 positive specimens confirmed by RIBA was 19.850 / 252%, the negative rate was 54.8% and 138 / 252%, and the uncertainty ratio was 25.44% / 64252%. All the 27 samples with positive nucleic acid test were ELISA for double reagent reactivity and positive for confirmatory test. There was significant difference between the results of RIBA confirmatory experiment and nucleic acid detection by ELISA reagent. Conclusion it is more safe to select two times of ELISA once to detect nucleic acid. For a high proportion of false positive specimens, a follow-up system should be established to preserve the blood donors to the maximum extent.
【作者单位】：贵州省血液中心;贵州医科大学基础学院免疫学教研室;
【基金】：贵州省卫生和计划生育委员会科学技术基金项目(GZWKJ2012-1-093)
【分类号】：R446.6;R512.63

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