培养福氏志贺菌感染大鼠回肠组织病理学的特点

发布时间：2018-02-09 14:35

本文关键词： 志贺菌痢疾结肠病理学组织工程实验动物消化系统损伤与修复模型福氏志贺菌感染远端回肠直肠组织病理　出处：《中国组织工程研究》2016年49期 　论文类型：期刊论文

【摘要】：背景:志贺氏菌属是引起细菌性痢疾的主要致病菌,细菌性痢疾是夏秋季节最常见的肠道传染病之一。目的:分析福氏志贺菌感染大鼠回肠组织的病理学特征。方法:同窝别清洁级SD雄性大鼠30只。随机分为非链霉素处理组、链霉素处理组、对照组,每组各10只。链霉素处理组50 g/L硫酸链霉素灌胃;非链霉素处理组用生理盐水灌胃;对照组不做任何处理。建立大鼠菌群紊乱模型后,非链霉素处理组、链霉素处理组用福氏志贺菌(9×108)CFU/m L灌胃建立大鼠痢疾模型,14 d后采用苏木精-伊红染色观察福氏志贺菌感染大鼠回肠组织的病理学特征。结果与结论:(1)福氏志贺菌感染大鼠远端回肠和直肠出现典型的菌痢病变,表现为黏膜出血、水肿,中性粒细胞浸润,杯状细胞破坏、出血,并出现疾病特征性的假膜性炎:黏膜表层坏死,有大量纤维素渗出,后者与坏死组织、红细胞、白细胞等一起构成假膜;脱落后的肠黏膜,形成形状不一的溃疡,溃疡浅表,很少超过黏膜下层;(2)各组大鼠出现症状时间、福氏志贺菌攻击前后大鼠体温值及体质量差异无显著性意义(P0.05);(3)结果说明,福氏志贺菌感染大鼠远端回肠和直肠租住出现菌痢典型的组织病理表现,可成功作为动物模型进行病因、发病机制和治疗等深入科研研究。
[Abstract]:Background: Shigella is the leading cause of bacillary dysentery. Bacillary dysentery is one of the most common intestinal infections in summer and autumn. Objective: to analyze the pathological characteristics of ileal tissue in rats infected with Shigella flexneri. Streptomycin treatment group, control group, 10 rats in each group. Streptomycin group 50 g / L streptomycin sulfate was given intragastric perfusion; non-streptomycin group was given normal saline; control group did not do any treatment. Non-streptomycin treatment group, In streptomycin treated group, Shigella flexneri 9 脳 10 8 CFU / mL was used to establish rat model of dysentery 14 days later, hematoxylin and eosin staining were used to observe the pathological characteristics of ileum of rats infected with Shigella flexneri. Results and conclusion: 1) Shigella flexneri. The distal ileum and rectum were infected with typical bacillary dysentery. Mucosal hemorrhage, edema, neutrophil infiltration, goblet cell destruction, bleeding, and characteristic pseudomembranous inflammation: mucosal surface necrosis, massive cellulose exudation, the latter associated with necrotic tissue, red blood cells, The white blood cells and so on form the pseudomembrane together; the intestinal mucosa after falling off, forms the ulcers of different shapes, the ulcers are superficial, rarely exceed the submucous membrane. There was no significant difference in body temperature and body mass between rats before and after Shigella flexneri attack. The results showed that the typical histopathological manifestations of bacillary dysentery were found in distal ileum and rectum of rats infected with Shigella flexneri. It can be successfully used as animal model for further scientific research, such as etiology, pathogenesis and treatment.
【作者单位】：昆明医科大学基础医学院病原生物学与免疫学系;昆明医科大学;昆明医科大学基础医学院神经科学研究所;
【基金】：云南省科技厅-昆明医科大学联合专项科研基金面上项目(2015FB008)~~
【分类号】：R516

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