桂林市经性传播的HIV-1的流行特征和亚型分析

发布时间：2018-02-21 17:25

本文关键词： HIV- 流行特征亚型 gag基因　出处：《安徽医科大学学报》2015年07期 　论文类型：期刊论文

【摘要】：目的了解桂林市2008年及以后确诊的经性传播的人类免疫缺陷病毒1型(HIV-1)感染者的流行病学及亚型的分布情况。方法选取样本100例,收集流行病学资料,采集抗凝全血,提取病毒RNA,RT-PCR法行gag基因扩增,对产物进行测序和分析。结果 100例样本中男女比例为2.125∶1;青壮年年龄组(20~49岁)患者为54%,50岁年龄组患者为45%;职业为农民的患者为40%,学生为6%;初中及以下文化水平患者占69%;经异性和同性性接触途径传播的分别为93%、7%。100例样本最终成功扩增得到61例样本的gag基因序列,CRF01-AE重组亚型48例,CRF07-BC重组亚型8例,B亚型5例。结论应更加重视婚检、孕检的艾滋病抗体筛查;关注老年人群经性接触途径感染艾滋病的现象;采取针对低素质人群的有效防治措施;随着主要传播途径和主要亚型的变化,迫切需要对高危人群进行宣传教育和行为干预以阻断HIV/AIDS的流行和传播。
[Abstract]:Objective to investigate the epidemiology and subtype distribution of sexually transmitted human immunodeficiency virus (HIV) 1 (HIV-1) infection in Guilin in 2008 and later. Methods 100 samples were collected to collect epidemiological data and collect anticoagulant whole blood. The gag gene was amplified by RT-PCR. Results the ratio of male to female in 100 samples was 2.125: 1; the adult age group was 2049.) the patient was 5450 years old, the patient was 45th; the farmer's occupation was 40th and the student was 6er; and the junior middle school and lower education level was affected. The gag gene sequence of 61 samples was successfully amplified, 48 cases of CRF01-AE recombinant subtype 8 cases of CRF07-BC recombinant subtype B, 5 cases of B subtype of CRF07-BC. Conclusion premarital examination should be paid more attention to. Screening of HIV antibodies for pregnancy tests; attention to the phenomenon of sexually transmitted HIV infection among the elderly; effective prevention and treatment measures for low-quality people; as the main transmission routes and major subtypes change, There is an urgent need for publicity, education and behavioural intervention among high-risk groups to block the spread and spread of HIV/AIDS.
【作者单位】：桂林医学院附属医院感染性疾病科;桂林市疾病预防控制中心;桂林市传染病医院;军事医学科学院微生物流行病研究所;广西医科大学公共卫生学院;临沂市沂水中心医院;
【基金】：国家自然基金(编号:81273137) 国家科技重大专项课题(编号:2012ZX10001-002) 广西本级技术研究与开发项目(桂财教[2012]98号) 桂林市科学研究与技术开发项目(编号:20130120-18) 广西壮族自治区研究生教育创新计划资助项目(编号:YCSZ2013101)
【分类号】：R512.91

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