中山地区1020例泌尿生殖道沙眼衣原体感染情况及耐药性分析

发布时间：2018-02-22 04:16

本文关键词： 泌尿生殖道沙眼衣原体耐药基因　出处：《广东医学》2017年09期 　论文类型：期刊论文

【摘要】：目的探讨中山地区泌尿生殖道沙眼衣原体(Chlamydia trachomatis)感染情况及对常用抗菌药物的耐药性。方法收集1 020例泌尿生殖道标本,核酸扩增法扩增沙眼衣原体MOMP基因进行电泳并测序鉴定;阳性临床菌株通过PCR产物电泳并测序检测四环素耐药质粒tet M基因、大环内酯类耐药相关23S rRNA基因、核糖体蛋白基因L4和L22以及喹诺酮类耐药基因gyr A。结果 1 020例泌尿生殖道标本检出沙眼衣原体感染者137例(感染率13.43%),其中女性126例(感染率17.00%),男性11例(感染率3.94%),女性检出率明显高于男性(P0.0001),感染人群主要集中于21~30岁的年轻女性,以宫颈分泌物和阴道分泌物标本检出率最高。137株沙眼衣原体临床株中检出tet M基因18株,喹诺酮类gyr A-QRDR基因有83丝氨酸(AGT)-异亮氨酸(ATT)点突变耐药株10株。有4株同时存在四环素类和喹诺酮类耐药基因。结论 20~30岁女性人群为沙眼衣原体感染的高危人群。中山地区沙眼衣原体耐药菌株以四环素类和喹诺酮类为主,且出现多重耐药现象,未发现大环内酯类耐药菌。
[Abstract]:Objective to investigate the infection of Chlamydia trachomatis( Chlamydia trachomatis) in the genitourinary tract of Zhongshan area and its resistance to common antimicrobial agents. Methods 1 020 specimens of genitourinary tract were collected, the MOMP gene of Chlamydia trachomatis was amplified by nucleic acid amplification and identified by sequencing. The tet M gene of tetracycline resistance plasmid and the 23s rRNA gene associated with macrolide resistance were detected by PCR product electrophoresis and sequencing. Ribosomal protein genes L4 and L22 and quinolone resistant gene gyr A. results 137 cases of Chlamydia trachomatis infection (infection rate 13.43%) were detected in 1020 specimens of genitourinary tract, including 126 cases of female (infection rate 17.00%) and 11 cases of male infection. The positive rate of female was significantly higher than that of male (P 0.0001). 18 strains of tet M gene were detected from cervical secretions and vaginal secretions from 18 clinical strains of Chlamydia trachomatis (Chlamydia trachomatis). In quinolones gyr A-QRDR gene, there are 10 strains of point mutation resistance of 83 serine AGTG-isoleucine-ATT gene and 4 strains of tetracycline and quinolone resistance genes. Conclusion the female population aged 20 to 30 years old is a high-risk person for chlamydia trachomatis infection. The resistant strains of Chlamydia trachomatis in Zhongshan area were mainly tetracycline and quinolones. And the phenomenon of multi-drug resistance was found, and no macrolide resistant bacteria were found.
【作者单位】：中山市人民医院检验医学中心;广州市第八人民医院传染病研究所;
【基金】：中山市卫生局科研基金资助项目(编号:J2012002) 中山市科技计划项目(编号:2015B1025)
【分类号】：R440;R691.3

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