中国2015年脊髓灰质炎实验室网络的运转与评价

发布时间：2018-02-22 18:40

本文关键词： 脊髓灰质炎实验室网络脊髓灰质炎病毒疫苗衍生脊髓灰质炎病毒　出处：《中国疫苗和免疫》2017年02期 　论文类型：期刊论文

【摘要】：目的评价中国(未包括香港、澳门特别行政区和台湾地区)2015年脊髓灰质炎(脊灰)实验室网络(Polio laboratory network,PLN)运转情况,为中国维持无脊灰状态提供病毒学依据。方法对中国急性弛缓性麻痹(Acute flaccid paralysis,AFP)病例监测信息报告管理系统中31个省(自治区、直辖市)报告的AFP病例和中国疾病预防控制中心(CDC)病毒病预防控制所国家脊灰实验室(National Polio Laboratory,NPL)的监测数据库进行统计分析,对PLN的各项运转指标进行评价。结果 2015年中国共报告5 218例AFP病例,PLN收到5 173例AFP病例的10 335份粪便标本;14天内双份粪便标本采集率为92.76%;合格粪便标本采集率为92.16%,病毒分离结果 14天内反馈率为96%。5 173例病例中,L20B阳性69例(1.33%);非脊灰肠道病毒(Non-polio enterovirus,NPEV)阳性472例(9.12%)。NPL收到省级脊灰实验室送检的分离自AFP病例、非AFP病例、AFP病例接触者及健康人的179株L20B细胞阳性分离物,其中173株为脊灰病毒(Poliovirus,PV),4株为NPEV,2株为非肠道病毒(Non-enterovirus,NEV)。173株PV中,8株为疫苗衍生脊灰病毒(Vaccine-derived poliovirus,VDPV),来自浙江、广东和辽宁;未发现脊灰野病毒(Wild poliovirus,WPV)。从脊灰病毒外环境监测采集的污水标本中分离到1株Ⅱ型VDPV。2015年NPL和31个省CDC脊灰实验室都通过了世界卫生组织(WHO)粪便标本脊灰等肠道病毒的新检测流程的能力验证标本考核。NPL和27个省CDC脊灰实验室通过了WHO型内鉴定能力验证考核。结论中国PLN 2015年运转正常,未发现WPV,为维持中国无脊灰状态,早期发现VDPV或VDPV病例,早期采取应对措施阻断其传播提供了重要的病毒学依据。
[Abstract]:Objective to evaluate the operation of Polio laboratory Network in 2015 in China (excluding Hong Kong, Macao Special Administrative region and Taiwan). Methods 31 provinces (autonomous regions) of acute flaccid paralysis (AFP) case monitoring information management system in China were used to provide virological basis for maintaining poliomyelitis free status. The surveillance database of AFP cases reported by the municipality directly under the Central Government and the National Polio Laboratory of the National Institute for Polio Disease Control and Prevention of the China Centers for Disease Control and Prevention (CDC) was statistically analyzed. Results in 2015, 5 218 cases of AFP were reported to have received 10 335 fecal specimens from 5 173 cases of AFP in China. The collection rate of double stool specimens in 14 days was 92.766.The rate of collection of qualified stool specimens was 92.76. For 92.16, the feedback rate in 14 days was 96.5 173 cases, 69 cases were positive for L20B, 472 cases were positive for non-poliomyelitis enterovirus.NPL received AFP cases from provincial polio laboratory. There are 179 strains of L20B cell positive isolates from non-AFP case contacts and healthy people, of which 173 strains are Poliovirusus PVV, 4 strains are NPEV virus, 2 strains are non-enterovirus.173 strains PV, 8 strains are vaccine-derived poliovirus vaccine-derived poliovirus virion, from Zhejiang, Guangdong and Liaoning. Wild poliovirus wild poliovirus was not found. One strain of NPL of type II VDPV.2015 year and 31 provincial CDC polio laboratories were isolated from waste water samples collected from external environment monitoring of poliovirus. All of them passed World Health Organization (WHO) fecal specimen polio and other enterovirus. NPL and 27 provincial CDC poliomyelitis laboratories passed the WHO type test. Conclusion in 2015, PLN in China operated normally. The absence of WPVprovides an important virological basis for maintaining poliomyelitis in China, early detection of VDPV or VDPV cases, and early response measures to block the transmission of WPVs.
【作者单位】：中国疾病预防控制中心病毒病预防控制所国家卫生和计划生育委员会医学病毒学和病毒病重点实验室世界卫生组织西太平洋区脊髓灰质炎参比实验室;浙江省疾病预防控制中心;中国疾病预防控制中心免疫规划中心;
【基金】：行使WHO西太区脊灰参比实验室职能,2015年国家卫生和计划生育委员会疾病控制专项
【分类号】：R512.4

【相似文献】