HBV感染与男性免疫性不育发生率的相关性分析

发布时间：2018-02-26 00:02

  本文关键词： 免疫性不育 HBV感染 血清HBV DNA 精浆HBV DNA　出处：《中华男科学杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的:探讨HBV感染与免疫性男性不育发生率的相关性。方法:筛选3 124例男性不育患者,依照血清乙肝表面抗原(Hbs Ag)结果将其分为HBV感染组和HBV未感染组。再通过免疫珠试验(IBT)测定抗精子抗体将免疫性不育患者又分为HBV感染和未感染的免疫性不育组。统计分析HBV感染和未感染免疫性不育组阳性率的差异、HBV感染和未感染免疫性不育组精液常规参数的差异、HBV感染不育患者血清和精浆HBV DNA拷贝数相关性、血清和精浆HBV DNA拷贝数与精液常规参数的相关性以及精浆HBV DNA拷贝数与免疫性不育发生率的差异。精子浓度、前向运动精子(PR)百分率用计算机辅助精子分析系统,精子形态检查用Diff-Quik染色法。HBs Ag检查用ELISA法,血清和精浆HBV DNA拷贝数用荧光定量PCR法。结果:HBV感染组和未感染组免疫性不育阳性率分别为20.3%和3.3%,差异有统计学意义(χ~2=187.5 P0.01)。HBV感染和未感染免疫性不育组精液量、精子浓度、PR之间差异无统计学意义(P0.05),而两组间正常精子形态百分率(MNS)分别为(3.9±1.7)%和(6.3±2.2)%、,差异有统计学意义(P0.05)。血清和精浆HBV DNA拷贝数存在正相关(rs=0.86,P0.01)。血清和精浆HBV DNA拷贝数与PR、MNS均呈负相关[(r=-0.233,P0.01和r=-0.465,P0.01)和(r=-0.250,P0.01和r=-0.508,P0.01)]。精浆HBV DNA不同拷贝数量级组免疫性不育阳性率差异无统计学意义(P0.05)。结论:HBV感染可增加免疫性不育的发生率,并与精子质量低下有相关性。
[Abstract]:Objective: to investigate the correlation between HBV infection and the incidence of immune male sterility. According to the results of serum hepatitis B surface antigen (HBs), the patients were divided into HBV infected group and HBV uninfected group, and the immune infertile patients were divided into HBV infected group and uninfected immune infertility group by detecting anti-sperm antibody by immunological bead test. The difference of semen routine parameters between HBV infected and uninfected immune infertility patients was analyzed statistically. The correlation between HBV DNA copy number in serum and seminal plasma of infertile patients with HBV infection was analyzed. The correlation between HBV DNA copy number in serum and seminal plasma and semen routine parameters, and the difference between seminal plasma HBV DNA copy number and the incidence of immune infertility. Sperm morphology was examined by Diff-Quik staining. HBs Ag was detected by ELISA. The copy number of HBV DNA in serum and seminal plasma was determined by fluorescence quantitative PCR. Results the positive rates of immunological infertility in the two groups were 20.3% and 3.3 respectively (蠂 2 187.5 P 0.01). There was no significant difference in sperm concentration and PR between the two groups (P 0.05), but the percentage of normal sperm morphology between the two groups was 3.9 卤1.7% and 6.3 卤2.2%, respectively. The difference was statistically significant (P 0.05). There was a positive correlation between serum and seminal plasma HBV DNA copy number. There was a positive correlation between serum and seminal plasma HBV DNA copy number. There was a negative correlation between the number and the number of PRNs. There was no significant difference in the positive rate of immunological sterility in the seminal plasma HBV DNA groups with different copy orders of magnitude. Conclusion the infection of HBV in seminal plasma can increase the incidence of immune infertility, and increase the incidence of immunological sterility in the seminal plasma of different copy orders of magnitude (P 0.01 and P 0.01, P 0.01, P 0.01). Conclusion the incidence of immunological infertility can be increased by infection of HBV in seminal plasma, and there is no significant difference in the positive rate of immunological sterility among the groups with different copies of seminal plasma HBV DNA. It was associated with low sperm quality.
【作者单位】： 宁波市中医院男科实验室;
【分类号】：R512.62;R698.2
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本文编号：1535677