2012年江苏部分地区动物中发热伴血小板减少综合征病毒携带状况调查

发布时间：2018-03-03 00:06

本文选题：发热伴血小板减少综合征病毒(SFTSV)　切入点：实时荧光RT-PCR　出处：《华中科技大学学报(医学版)》2015年02期 　论文类型：期刊论文

【摘要】：目的了解江苏部分地区动物中发热伴血小板减少综合征病毒(SFTSV)携带状况,探讨其可能的传播媒介和自然宿主。方法 2012年3月~12月在江苏省发现发热伴血小板减少综合征病例的7个市/县,采集鼠、牛、羊、刺猬、鸟类和蜱等动物标本,提取总核酸后,应用实时荧光RT-PCR法对标本进行布尼亚病毒S基因核酸的检测,阳性标本采用Vero细胞进行病毒分离。结果采集761只(管)动物,43只(管)检出SFTSV核酸阳性,阳性率为5.65%。采集3 728份标本,阳性49份,阳性率为1.31%。鼠的病毒携带率最高(6.84%),蜱标本阳性率最高(4.94%)。除蜱标本外的3 647份标本中检出阳性45份,阳性率为1.23%,不同器官阳性率分别为淋巴结(50.00%)、血清(2.34%)、脾(1.64%)、心(1.64%)、肝(1.28%)、肺(1.09%)、脑(0.94%)、肾(0.73%)、血块(0.41%)。5月标本阳性率最高(4.01%)。从1份羊血清及其体表1份蜱标本中各分离出1株SFTSV病毒。结论鼠、牛、羊和蜱均可携带SFTSV,可能是其自然宿主或传播媒介。
[Abstract]:Objective to investigate the prevalence of fever associated with thrombocytopenia syndrome virus (SFTSVV) in some animals in Jiangsu Province. Methods from March 2012 to December, 7 cities / counties with fever and thrombocytopenia syndrome were found in Jiangsu Province. The specimens of mice, cattle, sheep, hedgehog, birds and ticks were collected. After the total nucleic acid was extracted, the S gene nucleic acid of Bunia virus was detected by real-time fluorescence RT-PCR method. The positive samples were isolated by Vero cells. The positive rate was 5.65. 3 728 specimens were collected, 49 were positive, the positive rate was 1.31. The virus carrying rate of mice was the highest, and the positive rate of ticks was 4.94.The positive rate was 45 out of 3 647 specimens except ticks. The positive rate was 1.23. The positive rates of different organs were lymph nodes 50.000.34, the spleen 1.64, the heart 1.64, the liver 1.28g, the lung 1.09m, the brain 0.94g, the kidney 0.731.The highest positive rate was 4.010.The SFTSV virus was isolated from one sheep serum and one tick from the body surface on May. Cattle, sheep and ticks can carry SFTSVs, which may be natural hosts or vectors.
【作者单位】：江苏省疾病预防控制中心卫生部肠道病原微生物重点实验室;
【基金】：“十二五”国家科技重大专项计划(No.2012ZX10004-210-004) 江苏省“十二五”医学重点人才计划(No.H201118) 江苏省科教兴卫工程(No.ZX201109,JKRC2011001)
【分类号】：R510

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