中国脊髓灰质炎实验室网络开展细胞系支原体检测及现状分析

发布时间：2018-03-07 08:05

本文选题：脊髓灰质炎实验室网络　切入点：细胞系支原体检测　出处：《中国疫苗和免疫》2017年01期 　论文类型：期刊论文

【摘要】：目的通过对中国脊髓灰质炎(脊灰)实验室网络(China Polio Laboratory Network,CPLN)开展细胞系支原体检测(Cell mycoplasma detection,CMD),评价CMD在细胞质量控制中的作用,探讨其与脊灰病毒分离率的联系。方法采用巢式聚合酶链反应(Nested polymerase chain reaction,n PCR)法对CPLN常规使用的RD、L20B细胞系进行支原体检测。结果 2013年8月至2016年8月,在CPLN中除西藏外的30个省级脊灰实验室已正式开展CMD,并按时向国家脊灰实验室上报结果。CMD结果显示,30个省级脊灰实验室所用的每个批次的L20 B和RD细胞,支原体检测结果均为阴性。结论三年间CPLN 30个省级脊灰实验室用于脊灰病毒分离的细胞系均未出现支原体污染,细胞系质量正常且稳定;CMD作为CPLN细胞质量控制的重要组成部分,为CPLN运转质量提供可靠的数据支撑。
[Abstract]:Objective to evaluate the role of CMD in cell quality control by carrying out cell line detection of mycoplasma mycoplasma by using China Polio Laboratory Network (CPLN), a laboratory network of Chinese poliomyelitis (poliomyelitis). Methods the nested polymerase chain reactionn PCR method was used to detect mycoplasma in the RDD-L20B cell line, which was routinely used by CPLN. Results from August 2013 to August 2016, mycoplasma was detected by nested polymerase chain reactionn PCR. In addition to Tibet, 30 provincial polio laboratories in the CPLN have officially launched CMD.CMD reports the results to the National Polio Laboratory on time. The results show that L20B and Rd cells are used in each batch of 30 provincial polio laboratories. Conclusion No mycoplasma contamination was found in all of the cell lines used for poliovirus isolation in 30 provincial polio laboratories of CPLN during the past three years, and the quality of the cell lines was normal and stable as an important part of the quality control of CPLN cells. Provide reliable data support for CPLN operation quality.
【作者单位】：中国疾病控制中心病毒病预防控制所国家卫生和计划生育委员会医学病毒和病毒病重点实验室世界卫生组织西太平洋区脊髓灰质炎参比实验室;
【基金】：基金项目:2015年WHO西太区脊髓灰质炎参比实验室职能
【分类号】：R512.4

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