计划免疫前后出生的献血者HBV DNA阳性状况及其HBV感染的血清学和分子病毒学特征分析

发布时间：2018-03-07 19:19

  本文选题：乙型肝炎病毒　切入点：计划免疫　出处：《中国输血杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的了解深圳市在计划免疫前后出生的无偿献血者HBV感染情况,分析其血清学和分子生物学特征。方法将本中心2016年2-6月收集的26 320人(份)无偿献血者标本,以1992年为界分为计划免疫前出生组组:19 898人(份),年龄18-24岁;计划免疫后出生组:6 422人(份),24-55岁。分别将每组再分为HBs Ag(+)/HBV DNA(+)、HBs Ag(+)/HBV DNA(-)、HBs Ag(-)/HBV DNA(+)和HBs Ag(-)/HBV DNA可疑(NAT初筛阳性、鉴定试验阴性)4种类型,做"乙肝两对半"检测与HBV DNA小容量和大容量提取,采用巢氏PCR方法扩增BCP/PC和S区基因序列,并对所得序列做基因型分析,同时采用实时荧光定量PCR检测(q PCR)和分析。结果本组26 320(人)份献血者标本,通过酶免方法初筛检出242例HBs Ag不合格标本,经NAT、巢氏-PCR和q PCR检测HBV DNA阳性率为0.741%(195/26 320),其中195名HBV阳性者里有164(130+34)人为初次献血者。计划免疫前后出生2组中初次献血者的HBV DNA阳性率分别为1.309%(130/9 929)vs 0.707%(34/4 810)(P0.05);隐匿性乙肝感染(OBI)阳性率分别为0.256%(51/19 898)vs 0.093%(6/6 422)(P0.05)。在可分型的120例标本中,HBV基因型B型95例、C型25例,2种基因型在2组间(74/19 vs 21/6)的分布无明显差异(P0.05)。结论乙肝疫苗的接种明显降低了献血人群HBV感染的风险,有益于输血安全保障的提高。
[Abstract]:Objective to investigate the HBV infection in unpaid blood donors born before and after immunization in Shenzhen, and to analyze its serological and molecular biological characteristics. In 1992, there were 19 898 children (age 18-24) in the group of birth before the planned immunization, and 6 422 (24 to 55 years old) in the group of births after the planned immunization. Each group was subdivided into two groups: HBs Ag(-)/HBV (/ / HBDNA) and HBs Ag(-)/HBV DNA (HBs Ag(-)/HBV DNA), respectively. Four types of negative DNA were identified. "Hepatitis B two-and-half" detection and HBV DNA small volume and large volume extraction were performed. The gene sequences of BCP/PC and S region were amplified by nested PCR method, and genotypes were analyzed. At the same time, real-time fluorescence quantitative PCR was used to detect qPCRs and analysis. Results A total of 26 320 (human) blood donor samples were detected by enzyme immunoassay (Elisa), and 242 unqualified samples of HBs Ag were detected by enzyme immunoassay. The positive rate of HBV DNA detected by NATT, Nat's -PCR and Q PCR was 0.741- / 26,320g / 195, of which 164 / 13034 were first-time blood donors. The positive rates of HBV DNA were 1.309% / 9 929 vs 0.70734 / 4 810% P0.05 respectively in the two groups born before and after planned immunization. The positive rates of HBV infection OBI were 0.2566 / 19 898 vs 0.0932 / 6 / 6 / 6 422 / P0.05 respectively. There was no significant difference in the distribution of HBV genotype B genotype B genotype B type B genotype and B genotype B genotype in 25 patients with C genotype between the two groups (P 0.05). Conclusion Hepatitis B vaccine vaccination has a significant decrease in the distribution of HBV genotype B and B genotype in the two groups. Conclusion Hepatitis B vaccine vaccination has a significant decrease in the distribution of HBV genotype B genotype B and B genotype B genotype in 25 patients with C genotype. Conclusion the distribution of hepatitis B vaccine is significantly lower than that of the control group (P 0.05). Lower risk of HBV infection in blood donors, It is beneficial to improve the safety and security of blood transfusion.
【作者单位】： 深圳市血液中心;大连医科大学检验系;
【基金】：深圳市卫生计生系统科研项目(SZGW2017018)
【分类号】：R512.62
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本文编号：1580640