核酸定量检测应用于HIV-1感染诊断的研究

发布时间：2018-03-10 21:08

本文选题：型艾滋病病毒　切入点：核酸定性检测　出处：《中国艾滋病性病》2017年01期 　论文类型：期刊论文

【摘要】：目的探讨核酸定量检测在1型艾滋病病毒(HIV-1)感染诊断中的应用。方法选取云南省德宏州216例样本,包括136例HIV-1阳性母亲所生活产婴儿和80例HIV-1阳性成人,分别用HIV-1核酸定性和定量方法进行检测,综合对比分析2种方法的检测结果。结果 216例样本的核酸定性和定量检测试验一致性极好,53例样本(50例成人样本和3例婴儿样本)核酸阳性,血浆病毒载量5 000拷贝/mL。随访50例成人样本的蛋白印迹试验(WB),均为确证HIV-1抗体阳性。163例阴性样本(30例成人样本和133例婴儿样本)核酸阴性,血浆病毒载量低于检测下限。结论当血浆病毒载量5 000拷贝/mL,核酸定量检测试验对于HIV-1感染阳性样本是一种有效的实验室诊断方法。
[Abstract]:Objective to investigate the application of quantitative detection of nucleic acid in the diagnosis of HIV-1) infection. Methods 216 samples were collected from Dehong Prefecture, Yunnan Province, including 136 infants born in HIV-1 positive mothers and 80 HIV-1 positive adults. HIV-1 nucleic acid was detected by qualitative and quantitative methods. Results the qualitative and quantitative nucleic acid test of 216 samples was consistent with that of 53 samples, 50 adult samples and 3 infant samples were positive for nucleic acid. Plasma viral load was 5 000 copies / mL. The results of Western blot test of 50 adult samples were confirmed to be positive for HIV-1 antibody. 30 adult samples and 133 infant samples were confirmed to be nucleic acid negative. Conclusion when plasma viral load is 5 000 copies / mL, nucleic acid quantitative assay is an effective method for laboratory diagnosis of HIV-1 infection positive samples.
【作者单位】：德宏州疾病预防控制中心性病艾滋病防制科;中国疾病预防控制中心性病艾滋病预防控制中心参比室;
【基金】：国家重点地区艾滋病防治项目国家科技重大专项(2013zx10004-906)~~
【分类号】：R512.91

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