CBS对红色毛癣菌的抗菌及协同增效作用研究

发布时间：2018-03-14 04:31

本文选题：红色毛癣菌　切入点：CBS　出处：《中国新药杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的:研究荧光增白剂CBS(flourescent brightener CBS,CBS)对红色毛癣菌的抗菌作用,以及联合使用酮康唑和特比萘芬的增效作用。方法:采用滤纸片扩散法,探究CBS对红色毛癣菌的单一抗菌作用;采用MTT法探究CBS联合酮康唑、特比萘芬对红色毛癣菌的协同抗菌作用;然后采用均匀设计方法结合SPSS软件建立模拟方程,并通过验证试验确定最优配方;用豚鼠构建红色毛癣菌感染的动物模型,评价最优配方的实际药用效果。结果:酮康唑、特比萘芬联合CBS均对其存在协同作用。均匀设计得到最优配方是CBS浓度为1.861 mg·mL~(-1),酮康唑浓度为1.5μg·mL~(-1),特比萘芬浓度为0.175μg·mL~(-1),SDS浓度为0.077 4 mg·mL~(-1),氮酮浓度为6 mg·mL~(-1)时,理论最大抑制率为75.34%。进行动物试验表明联合药物比单一药物平均缩短了6 d的治疗时间。结论:CBS对红色毛癣菌有抗菌作用,联合酮康唑和特比萘芬对红色毛癣菌具有协同增效作用。
[Abstract]:Objective: to study the antibacterial effect of CBS(flourescent brightener CBSCBS against Trichophyton rubrum and the synergistic effect of ketoconazole and terbinafine. Methods: the single antibacterial effect of CBS on Trichophyton rubrum was studied by filter paper diffusion method. The synergistic antibacterial effect of CBS combined with ketoconazole and terbinafine against Trichophyton rubrum was investigated by MTT method. The animal model of Trichophyton rubrum infection in guinea pigs was established to evaluate the actual medicinal effect of the optimal formulation. Results: ketoconazole, ketoconazole, ketoconazole, ketoconazole, The optimal formulation of terbinafine combined with CBS was 1.861 mg 路mL ~ (-1) of CBS, 1.5 渭 g 路mL ~ (-1) of ketoconazole, 0.175 渭 g 路mL ~ (-1) of terbinafine and 0.0774 mg 路mL ~ (-1) of CBS, and 6 mg 路mL ~ (-1) of azone, respectively, and the optimum formula was as follows: the concentration of CBS was 1.861 mg 路mL ~ (-1), the concentration of ketoconazole was 1.5 渭 g 路mL ~ (-1), the concentration of terbinafine was 0.175 渭 g 路mL ~ (-1), the concentration of terbinafine was 0.077 4 mg 路mL ~ (-1). The theoretical maximum inhibitory rate was 75.34. Animal experiments showed that the combined drug had an average treatment time of 6 days shorter than that of a single drug. Conclusion: CBS has antimicrobial effect against Trichophyton rubrum. The combination of ketoconazole and terbinafine has synergistic effect on Trichophyton rubrum.
【作者单位】：重庆理工大学药学与生物工程学院;山西同达药业有限公司;
【分类号】：R756

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