2012-2013年毕节市流行性感冒病毒流行趋势分析研究

发布时间：2018-03-16 16:25

本文选题：流行性感冒　切入点：病毒　出处：《中华医院感染学杂志》2015年13期 　论文类型：期刊论文

【摘要】：目的分析2012-2013年毕节市流行性感冒病毒培养及鉴定结果,为临床提供准确的流行性感冒病毒感染诊断和治疗依据。方法于2012-2013年对国家级流行性感冒样病例监测哨点医院采集送检的流行性感冒样病例的鼻咽拭子,进行Real-time PCR流行性感冒病毒核酸检测,如为PCR核酸阳性结果,则采用狗肾上皮细胞(MDCK)培养法分离及鉴定流行性感冒病毒。结果 2012-2013年毕节市流行性感冒病毒监测中心共收到送检的流行性感冒样病例监测标本1 437份,经PCR流行性感冒病毒核酸检测出阳性281份,阳性率19.6%;培养及鉴定出流行性感冒病毒31株,培养阳性率11.0%,其中2012年2月-2013年1月流行性感冒病毒核酸检测阳性104份,共培养及鉴定出流行性感冒病毒9株,培养阳性率8.7%,9株流行性感冒病毒鉴定分型,BY流行性感冒病毒1株、BV流行性感冒病毒3株、H3N2亚型流行性感冒病毒5株;2013年2月-2014年1月流行性感冒病毒核酸检测阳性177份,共培养及鉴定出流行性感冒病毒22株,培养阳性率12.4%,22株流行性感冒病毒鉴定分型,BY流行性感冒病毒12株、新H1N1亚型流行性感冒病毒10株。结论 2012年2月-2013年1月毕节市流行性感冒病毒以H3N2亚型和B型流行为主,2013年2月-2014年1月毕节市流行性感冒病毒以BY和新H1N1亚型流行为主,其中B型流行性感冒病毒为这两年的流行毒株。
[Abstract]:Objective to analyze the results of culture and identification of influenza virus in Bijie city from 2012 to 2013. Methods the nasopharynx swabs of influenza like cases collected from sentinel hospitals of national level were collected from 2012 to 2013 to provide accurate basis for the diagnosis and treatment of influenza virus infection. To detect Real-time PCR influenza virus nucleic acid, such as the positive result of PCR nucleic acid, The influenza virus was isolated and identified by MDCK culture method. Results 1 437 samples were collected from Bijie City Influenza virus Surveillance Center in 2012-2013. The results showed that there were 281 positive strains of influenza virus nucleic acid detected by PCR, the positive rate was 19.60.31 strains of influenza virus were cultured and identified, and the positive rate of culture was 11.0%, of which 104 samples were positive for influenza virus nucleic acid from February 2012 to January 2013. 9 strains of influenza virus were co-cultured and identified. The positive rate of culture was 8. 7%. 9 strains of influenza virus were identified and typed. One strain of B virus, 3 strains of influenza virus, 3 strains of H3N2 subtype influenza virus, and 177 positive strains of nucleic acid of influenza virus were detected from February 2013 to January 2014. A total of 22 strains of influenza virus were cultured and identified. The positive rate of culture was 12.4% and 22 strains of influenza virus were identified and typed. 12 strains of influenza virus were identified. Conclusion from February 2012 to January 2013, H3N2 subtype and B type of influenza virus were prevalent in Bijie city, and those of by and H1N1 subtype were predominant in February 2013 to February 2013 to February 2013 to February 2013 to February 2013 to February 2013 to February 2013 to February 2013 to February 2013 to February 2013 to February 2013 to February 2013 to February 2013 to February 2013 to February 2013 to February 2013 to February 2013 to February 2013. Type B influenza virus is the epidemic strain of the last two years.
【作者单位】：毕节市疾病预防控制中心检验科;毕节市第一人民医院检验科;
【基金】：贵州省重大公共卫生基金项目(2100409)
【分类号】：R511.7

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