青海省2013年手足口病病原特征和人肠道病毒A组71型基因特征

发布时间：2018-03-25 10:54

本文选题：手足口病　切入点：人肠道病毒A组型　出处：《中国疫苗和免疫》2015年01期

【摘要】：目的对青海省2013年手足口病(Hand,Foot and Mouth Disease;HFMD)的病原谱和人肠道病毒A组71型(Human Enterovirus Group A Type 71,HEV_(A71))的基因特征进行分析。方法对青海省2013年送检的HFMD标本,用荧光定量逆转录-聚合酶链反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)方法进行筛查,对HEV_(A71)阳性标本进行病毒分离,对分离到的病毒提取核酸,用RT-PCR方法对VP1编码区进行扩增及核苷酸序列测定和整理分析,并与HEV_(A71)各基因型和基因亚型的代表株序列构建亲缘关系进化树。结果对青海省2013年分离的62株HEV_(A71)进行VP1编码区核苷酸序列测定后,分析发现它们在VP1编码区核苷酸水平和氨基酸水平的同源性分别为95.5%~100.0%和99.0%~100.0%。与其他各基因型和基因亚型的HEV_(A71)代表株构建的亲缘进化树中显示,青海省HEV_(A71)分离株与C4亚型代表株聚为一簇,属于C4基因亚型C_(4a)进化分支。与2008年、2012年在青海省分离的HEV_(A71)在核苷酸和氨基酸水平的同源性分别为95.8%~97.5%和99.0%~99.7%。结论2013年,C_(4a)亚型HEV_(A71)在青海省持续传播并占有绝对优势,且存在两条传播链的流行。
[Abstract]:Objective to analyze the pathogenic spectrum of hand foot and mouth disease (HFMD) in 2013 and the genetic characteristics of human Enterovirus Group A Type 71 (HEV A71A). Methods HFMD samples from Qinghai Province in 2013 were analyzed. Reverse Transcription-Polymerase Chain reaction polymerase chain reaction (reverse Transcription-Polymerase Chain RT PCR) method was used to screen the positive specimens of HEV StackA71, and to extract the nucleic acid from the virus. The coding region of VP1 was amplified by RT-PCR, and the nucleotide sequence was sequenced and analyzed. The phylogenetic tree was constructed with the representative strains of each genotype and gene subtype. Results the nucleotide sequences of the VP1 coding region of 62 HEV / A71 strains isolated in 2013 in Qinghai Province were determined. It was found that the homology of nucleotide level and amino acid level in VP1 coding region were 95.5% and 99.00.0%, respectively. In Qinghai Province, HEVSHAN A71) the isolates and C4 subtype representative strains were clustered into a cluster. The homology of HEVS A71) at nucleotide and amino acid levels was 95.87.5% and 99.00.0.Conclusion in 2013, HEVART A71), a subtype of C _ 4 gene subtype, continued to spread and had an absolute advantage in Qinghai Province, and the homology was 95.8g / 97.5% and 99.00.Conclusion in 2013, HEVS-1) subtype of C _ 4 gene subtype was continuously transmitted and had an absolute advantage in Qinghai Province. And there are two transmission chains of popularity.
【作者单位】：中国疾病预防控制中心病毒病预防控制所卫生部医学病毒学和病毒病重点实验室世界卫生组织西太平洋区脊髓灰质炎参比实验室;青海省疾病预防控制中心;
【基金】：国家重点基础研究发展计划(973计划)重要病原体变异规律与致病机制研究子课题(编号:2011CB504902) 十二五国家科学技术艾滋病和病毒性肝炎等重大传染病防治重大专项病毒性传染病病原谱流行规律及变异研究课题(编号:2013ZX10004202)
【分类号】：R512.5

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