蓝氏贾第鞭毛虫诊断

发布时间：2018-03-26 15:35

本文选题：蓝氏贾第鞭毛虫　切入点：直接镜检　出处：《中国比较医学杂志》2015年01期

【摘要】：目的蓝氏贾第鞭毛虫作为一种重要的人兽共患寄生虫病贾第虫病的病原,其对SPF实验动物质量造成的潜在威胁不容忽视。本研究的目的是建立蓝氏贾第鞭毛虫快速诊断方法,并对17个生产厂家516批2562只SPF实验动物检查结果进行分析。方法用直接镜检法、快速姬姆萨染色法和多重PCR方法,对蓝氏贾第鞭毛虫进行检测。结果在SPF实验动物中检出数量众多的蓝氏贾第鞭毛虫滋养体和包囊,鉴定出蓝氏贾第鞭毛虫18S r DNA、β-giardin、TPI和GDH基因。直接镜检法、快速姬姆萨染色法和多重PCR方法均能检出蓝氏贾第鞭毛虫。17个生产厂家516批2562只SPF实验动物蓝氏贾第鞭毛虫阳性检出率22.9%(586/2562)。结论直接镜检法、快速姬姆萨染色法和多重PCR方法具有高度的敏感性和特异性,可用于蓝氏贾第鞭毛虫的快速诊断。17个生产厂家516批SPF实验动物蓝氏贾第鞭毛虫检查结果未能全部符合规定。
[Abstract]:Objective as an important pathogen of zoonotic parasitic giardiosis, Giardia lamblia can not be ignored as a potential threat to the quality of SPF laboratory animals. The aim of this study is to establish a rapid diagnostic method for Giardia lamblia. The results of 2562 SPF laboratory animals in 516 batches from 17 manufacturers were analyzed. Methods Direct microscopic examination, rapid Giemsa staining and multiple PCR methods were used. Results A large number of Giardia lamblia trophozoites and cysts were detected in SPF laboratory animals, and 18s r DNA, 尾 -giardinine TPI and GDH genes of Giardia lamblia were identified. Giardia lamblia can be detected by rapid Giemsa staining and multiple PCR methods. The positive rate of Giardia lamblia was detected by 516 batches of 2562 SPF laboratory animals in 17 manufacturers. Conclusion the positive rate of Giardia lamblia is 22. 9% / 2562%. Rapid Giemsa staining and multiplex PCR are highly sensitive and specific, and can be used for rapid diagnosis of Giardia lamblia. The results of 516 batches of SPF laboratory animals from 17 manufacturers failed to meet the requirements.
【作者单位】：中国食品药品检定研究院;
【基金】：国家科技支撑课题(2013BAK11B01)
【分类号】：R531.7

【共引文献】