黄芪注射液联合恩替卡韦对乙型肝炎模型小鼠血清炎症因子及肝功能的影响

发布时间：2018-03-26 22:27

本文选题：黄芪注射液　切入点：恩替卡韦　出处：《中国药房》2017年34期

【摘要】：目的:研究黄芪注射液联合恩替卡韦对乙型肝炎模型小鼠血清炎症因子及肝功能的影响。方法:将60只小鼠随机分为正常对照组(生理盐水)、模型组(生理盐水)、黄芪注射液组(0.5 mL/10 g)、恩替卡韦组(45μg/kg)和联用组(0.5 mL/10 g黄芪注射液+45μg/kg恩替卡韦),各12只。除正常对照组外,其余4组小鼠均建立乙型肝炎模型。成模后,各组小鼠ig给药,每天1次,连续4周。给药结束后,检测小鼠血清中肿瘤坏死因子α(TNF-α)、干扰素γ(IFN-γ)、白细胞介素8(IL-8)、天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、总胆红素(TBIL)水平,观察各组小鼠肝组织的病理学变化。结果:与正常对照组比较,模型组小鼠血清中TNF-α、IFN-γ、IL-8、AST、ALT和TBIL水平均显著升高(P0.05),肝细胞呈弥散性重度脂肪变性。与模型组比较,各给药组小鼠血清中TNF-α、IFN-γ、IL-8、AST、ALT和TBIL水平均显著降低(P0.05),肝组织病理学变化得到不同程度改善,且联用组小鼠指标改善程度优于黄芪注射液组和恩替卡韦组(P0.05)。结论:黄芪注射液联合恩替卡韦有助于下调乙型肝炎模型小鼠血清中炎症因子表达、改善肝功能水平,且两药联用效果优于两药单用。
[Abstract]:Objective: to study the effect of astragalus injection combined with entecavir on serum inflammatory factors and liver function in mice with hepatitis B. methods: 60 mice were randomly divided into normal control group (normal saline group) and model group (normal saline group). Astragalus injection group, astragalus injection group (n = 12), entecavir group (n = 45 渭 g / kg) and combined group (n = 12, n = 12). The other four groups of mice established hepatitis B model. After the model was established, mice in each group were given intravenously once a day for 4 weeks. The levels of TNF- 伪, IFN- 纬, IL-8, aspartate aminotransferase, alanine aminotransferase (alt) and total bilirubin (TBIL) in serum of mice were measured. The serum levels of TNF- 伪 -IFN- 纬 -IFN- 纬 -IFN- 纬 -IL-8 and TBIL in serum of mice in the model group were significantly higher than those in the control group, while the levels of alt and TBIL in serum of the model group were significantly higher than those of the model group. Compared with the model group, the serum levels of TNF- 伪 IFN- 纬 -IFN- 纬 -IL-8IL-8, ASTTalt and TBIL were significantly decreased, and the pathological changes of liver tissue were improved in varying degrees. The index of mice in combination group was better than that of astragalus injection group and entecavir group (P 0.05). Conclusion: Astragalus injection combined with entecavir can down-regulate the expression of inflammatory factors in serum of mice with hepatitis B and improve the level of liver function. The effect of combined use of two drugs was better than that of two drugs alone.
【作者单位】：重庆三峡医药高等专科学校中医系;湖南中医药大学第一附属医院内科;
【基金】：重庆市教委科学技术研究项目(No.KJ121802)
【分类号】：R-332;R512.62

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