汉防己甲素对伊曲康唑、伏立康唑和泊沙康唑抗烟曲霉体外增效活性研究

发布时间：2018-03-27 04:40

本文选题：汉防己甲素　切入点：伊曲康唑　出处：《中国人兽共患病学报》2015年05期

【摘要】：目的探讨汉防己甲素(tetrandrine,TET)对伊曲康唑(itraconazole,ITR)、伏立康唑(voriconazole,VRC)和泊沙康唑(posaconazole,PCZ)体外抗烟曲霉有无增效活性。方法参照M38-A2方案的微量稀释法,以23株烟曲霉临床株为研究对象,测定TET对其的最大非细胞毒性剂量和ITC、VRC、PCZ单独及联合TET对其的最小抑菌浓度(minimal inhibitory concentrations,MICs)。结果 TET抗烟曲霉的最大非细胞毒性剂量为80μg/mL。ITC单独及联合TET对烟曲霉的MIC值范围分别为0.5μg/mL~32μg/mL和0.0625μg/mL~4μg/mL,差异有统计学意义(P0.05);VRC单独及联合TET的MIC值范围分别为0.25μg/mL~2μg/mL和0.03125μg/mL~0.25μg/mL,差异有统计学意义(P0.05);PCZ单独及联合TET的MIC值范围分别为0.125μg/mL~1μg/mL和0.03125μg/mL~0.25μg/mL,差异有统计学意义(P0.05)。结论汉防己甲素在体外对伊曲康唑、伏立康唑和泊沙康唑抗烟曲霉有增效活性。
[Abstract]:Objective to investigate the synergistic effect of Tetrandrine tetrandrine (TET) against Aspergillus fumigatus in vitro with itraconazoledol, voriconazoleine (VRC) and posaconazoledol (PCZs) in vitro. Methods 23 clinical strains of Aspergillus fumigatus were studied by microdilution method of M38-A2 regimen. The maximum non-cytotoxic dose of TET and the minimum inhibitory concentration of TET alone or in combination with TET were determined. Results the maximum non-cytotoxic dose of TET against Aspergillus fumigatus was 80 渭 g/mL.ITC alone and in combination with TET in the MIC range of Aspergillus fumigatus. The MIC values of TET alone and TET were 0.25 渭 g/mL~2 渭 g/mL and 0.03125 渭 g/mL~0.25 渭 g / mL, respectively. The MIC ranges of TET alone and TET were 0.125 渭 g/mL~1 渭 g/mL and 0.03125 渭 g/mL~0.25 渭 g / mL, respectively. Tetrandrine was used to treat itraconazole in vitro. Volconazole and posaconazole have synergistic activity against Aspergillus fumigatus.
【作者单位】：暨南大学附属第一医院;暨南大学真菌病研究所;广州医学院附属深圳沙井医院皮肤科;
【基金】：国家自然科学基金(81171542/81471995)~~
【分类号】：R519

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