HIV抗体确证试验不确定结果的解析

发布时间：2018-03-29 13:56

本文选题：艾滋病病毒抗体不确定　切入点：蛋白印迹试验带型　出处：《中国艾滋病性病》2017年07期

【摘要】：目的分析艾滋病病毒(HIV)抗体不确定者的检测结果,探讨HIV抗体不确定结果出现的规律和HIV不确定人群蛋白印迹试验(WB)带型的变化特征。方法对驻马店市HIV确证实验室2013-2015年报告的55例HIV抗体不确定人群进行跟踪随访,回顾性研究其WB带型及HIV酶联免疫吸附试验(ELISA)的S/CO值特征,观察其最终临床转归过程中的发展和变化。结果 2013-2015年驻马店地区共检测HIV抗体不确定者55例。经随访检测,有30例(54.5%)确证为阳性,21例(38.2%)确证阴性,4例(7.3%)失访。WB条带的组合模式共有18种。单次带型分析显示,p24带型出现26次,占总条带数的23.0%;其次是gp160,占总带数的19.5%。HIV筛查试验ELISA不同S/CO值样本的阳转率差异有统计学意义(χ~2=16.259,P=0.006)。结论驻马店市近几年发现HIV不确定结果中,env带的出现及HIV筛查试验ELISA的S/CO6.0预示HIV感染的可能性较大,对HIV感染的判断应结合流行病学史及实验室相关检测手段进行综合诊断。
[Abstract]:Objective to analyze the results of HIV / AIDS antibody detection in patients with uncertain HIV antibody. To investigate the regularity of the uncertain results of HIV antibody and the changing characteristics of the HIV HIV uncertain population. Methods 55 cases of HIV antibody uncertain population reported by HIV confirmatory laboratory in Zhumadian City from 2013 to 2015 were followed up. The characteristics of WB band type and S/CO value of HIV enzyme-linked immunosorbent assay (Elisa) were studied retrospectively, and the development and changes in the final clinical outcome were observed. Results 55 cases of uncertain HIV antibody were detected in Zhumadian area from 2013 to 2015. There were 18 kinds of combination patterns of the missing .WB bands. The single band analysis showed that the p24 band type appeared 26 times, and the P24 band pattern appeared 26 times by single band analysis, which was confirmed to be positive in 21 cases (38.2%) and confirmed negative in 4 cases (7. 3%). The positive conversion rate of ELISA samples with different S/CO values was statistically significant (蠂 ~ 2 / 2 ~ (16.259) P ~ (0.006)). Conclusion in Zhumadian City in recent years, the occurrence of HIV band and the HIV screening test were found in the uncertain results of HIV in the next place, gp160, and the positive transition rate of different S/CO samples (蠂 ~ 2 ~ 2 ~ (2) ~ (2) ~ (25) 9) (蠂 ~ (2) ~ (2)) (P ~ (0.006)). The S/CO6.0 of ELISA indicates the possibility of HIV infection. The diagnosis of HIV infection should be combined with epidemiological history and laboratory methods.
【作者单位】：驻马店市疾病预防控制中心;河南省疾病预防控制中心性病艾滋病防治研究所;
【基金】：河南省基础与前沿技术研究课题(162300410123) 河南省医学科技攻关计划项目(201602311)~~
【分类号】：R512.91

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