活动性结核患者单核细胞中B淋巴细胞诱导成熟蛋白1的mRNA水平降低

发布时间：2018-03-30 06:12

本文选题：结核分枝杆菌　切入点：活动性结核　出处：《细胞与分子免疫学杂志》2015年07期

【摘要】：目的研究活动性结核患者单核细胞中B淋巴细胞诱导成熟蛋白1(Blimp-1)的mRNA水平与结核分枝杆菌(MTB)潜伏感染者和健康者相比是否发生改变。方法收集活动性结核患者、结核菌潜伏感染者和和健康者的抗凝外周血,从外周血中分离并纯化单核细胞,荧光定量PCR检测单核细胞中Blimp-1 mRNA的水平。而后分别用MTB的H37Rv菌株全菌裂解液和ESAT-6/CFP-10的抗原多肽刺激健康者的单核细胞,荧光定量PCR检测刺激前后单核细胞中Blimp-1 mRNA的水平。结果与健康者相比,活动性结核患者、潜伏感染者单核细胞Blimp-1的mRNA水平显著降低;与未刺激的单核细胞相比,全菌裂解液刺激后的单核细胞Blimp-1的mRNA水平显著降低,而ESAT-6/CFP-10抗原多肽刺激后的单核细胞中Blimp-1的mRNA水平无显著变化。结论活动性结核患者和MTB潜伏感染者单核细胞Blimp-1的mRNA水平降低,MTB全菌裂解液刺激的人单核细胞中Blimp-1的mRNA水平降低。
[Abstract]:Objective to study whether the mRNA level of B lymphocyte inducible maturation protein (Blimp-1) in monocytes of active tuberculosis patients changed compared with that of latent infection of Mycobacterium tuberculosis and healthy people. Methods the patients with active tuberculosis were collected. The anticoagulant peripheral blood of latent infection of tuberculous bacilli and healthy persons were isolated and purified monocytes from peripheral blood. Fluorescence quantitative PCR was used to detect the level of Blimp-1 mRNA in monocytes, and then the monocytes of healthy subjects were stimulated with the whole bacterial lysate of H37Rv strain of MTB and the antigenic polypeptide of ESAT-6/CFP-10, respectively. Fluorescence quantitative PCR was used to detect the level of Blimp-1 mRNA in monocytes before and after stimulation. Results compared with healthy controls, the mRNA level of Blimp-1 in active TB patients was significantly lower than that in unstimulated monocytes. The mRNA level of Blimp-1 in monocytes stimulated by whole bacteria lysate was significantly decreased. There was no significant change in mRNA level of Blimp-1 in monocytes stimulated by ESAT-6/CFP-10 antigenic peptides. Conclusion the level of Blimp-1 mRNA in monocytes of active tuberculosis patients and latent patients with MTB decreased the mRNA level of Blimp-1 in monocytes stimulated by MTB-lysate.
【作者单位】：解放军第309医院全军结核病研究所全军结核病防治重点实验室结核病诊疗新技术北京市重点实验室;
【基金】：国家自然科学基金(81071318)
【分类号】：R52

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