结核感染酶联免疫法在结核病临床诊断中应用价值研究

发布时间：2018-04-10 10:06

本文选题：结核病　切入点：结核感染酶联免疫法　出处：《中华医院感染学杂志》2015年06期

【摘要】：目的探讨结核诊断中应用结核感染酶联免疫法(TB-IGRA)诊断的临床应用价值,为临床诊断提供参考。方法选择2012年1月-2013年12月60例疑似结核病患者,对其进行结核菌素试验(TST)及TB-IGRA试验,统计两种诊断方式结果,对比两种诊断方法敏感度、特异度、阴性似然比(LR-)、阳性似然比(LR+)。结果 60例疑似结核病患者TST阳性36例、阴性24例,结核分枝杆菌培养阳性37例、阴性23例,TB-IGRA阳性45例、阴性15例,结核分枝杆菌培养阳性48例、阴性12例;TB-IGRA灵敏度、特异度、假阳性率、假阴性率分别为91.67%、91.67%、8.33%、8.33%,均明显优于TST 70.27%、56.52%、43.48%、29.73%,对比差异均有统计学意义(P0.05);TB-IGRA诊断LR-为0.09、LR+为11.00。结论相比于TST试验、TB-IGRA试验诊断结核敏感性更好,且诊断快速,适合于结核筛检,应积极推广应用。
[Abstract]:Objective to explore the clinical application value of TB-IGRAs in the diagnosis of tuberculosis infection by enzyme linked immunosorbent assay (Elisa), and to provide reference for clinical diagnosis.Methods 60 suspected tuberculosis patients from January 2012 to December 2013 were selected and tested with tuberculin test (TST) and TB-IGRA test. The results of the two diagnostic methods were compared, and the sensitivity and specificity of the two diagnostic methods were compared.The negative likelihood ratio was lower than that of LR-G, and the positive likelihood ratio was lower than that of LR.Results among 60 suspected tuberculosis patients, 36 were positive for TST, 24 were negative, 37 were positive for mycobacterium tuberculosis, 45 were positive for TB-IGRA, 15 were negative, 48 were positive for mycobacterium tuberculosis and 12 were negative for TB-IGRA.The false positive rate and false negative rate were 91.67 and 9.33 and 8.33, respectively, which were significantly better than that of TST 70.27 and 56.52 and 43.48 and 29.733.The differences were statistically significant (P 0.05 and TB-IGRA = 0.09LR = 11.00).Conclusion compared with TST test, TB-IGRA test is more sensitive and rapid in diagnosis of tuberculosis. It is suitable for tuberculosis screening and should be popularized and applied.
【作者单位】：富阳市人民医院检验科;
【基金】：浙江省卫生高层次创新人才培养工程基金资助项目(2012-241)
【分类号】：R52

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