2007-2012年乐山地区HIV-1抗体阳性标本蛋白免疫印迹带型分析

发布时间：2018-04-10 14:01

本文选题：HIV抗体 + 蛋白印迹　；参考：《现代预防医学》2015年18期

【摘要】：目的了解乐山地区2007-2012年HIV-1抗体阳性标本蛋白免疫印迹带型分布情况,为临床诊断和治疗提供科学依据。方法采用WESTERN BLOT免疫印迹技术进行检测并严格按照《全国艾滋病检测技术规范》2009年修订版和试剂说明书要求进行实验操作和结果判断,使用SPSS 11.5进行统计处理。结果 1 163例确证阳性标本中除gag蛋白带型p55和p17阳性率较低外,其余带型阳性率均在94.00%以上;同时全带型组合出现率最高(57.70%);不同性别与蛋白印迹带型比较发现单条带型p55(χ2=7.427,P=0.006)和p17(χ2=6.370,P=0.012)在男女性别中的出现率差异有统计学意义;不同年龄组间单条带型gp41(χ2=8.827,P=0.032)、p51(χ2=11.418,P=0.010)、p55(χ2=16.622,P=0.001)出现率差异有统计学意义;在不同样品来源间的带型p31(χ2=14.662,P=0.023)、p55(χ2=18.594,P=0.005)出现率差异有统计学意义。结论乐山市HIV感染处于快速增长期,通过HIV-1抗体WESTERN BLOT带型分布在不同性别、不同年龄、不同人群中的带型分析,可对乐山市今后HIV感染者病原检测、诊断、传播源的追踪以及治疗提供有力的依据。
[Abstract]:Objective to investigate the distribution of Western blotting patterns in HIV-1 antibody positive specimens from 2007 to 2012 in Leshan area, and to provide scientific basis for clinical diagnosis and treatment.Methods WESTERN BLOT Western blot technique was used to detect the results. The experimental operation and result judgment were carried out in accordance with the revised edition of the 2009 revised edition of the National AIDS Test Technical Standard and the reagent specification. SPSS 11.5 was used for statistical treatment.Results (1) the positive rates of gag band type p55 and p17 were over 94.00% in all the confirmed positive specimens, except for the lower p55 and p17 positive rates.There was significant difference in the occurrence rate of p31 (蠂 ~ 2 ~ 2 ~ (14. 662) P ~ (1)) ~ (0.023) P ~ (55) (蠂 ~ (2) ~ (2)) ~ (18.594) P ~ (+) ~ (0.005)) among different samples.Conclusion HIV infection in Leshan City is in rapid growth stage. The analysis of HIV-1 antibody WESTERN BLOT banding pattern in different sex, different age and different population can detect and diagnose the pathogen of HIV infection in Leshan City in the future.Tracking of the source of transmission and treatment provide a powerful basis.
【作者单位】：四川省乐山市疾病预防控制中心;
【分类号】：R512.91;R446.6

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