2008-2012年江西省乙型流感病毒HA1基因特性分析

发布时间：2018-04-13 04:36

本文选题：B型流感病毒 + 分离株　；参考：《现代预防医学》2015年05期

【摘要】：目的了解2008-2012年江西省分离的B型流感病毒株HA1基因变异特征。方法采集监测医院和疑似流感疫情的流感样病例鼻咽拭子标本进行流感病毒分离,对分离到的B型流感病毒进行核酸的提取,采用逆转录-聚合酶链反应(RT-PCR)扩增病毒基因后进行核苷酸序列测定,用DNAStar5.0、Mage对测序结果进行分析处理,推导出氨基酸序列,进行基因特性分析。结果 10株Yamagata系B型毒株HA1基因之间的同源性为97.5%~100.0%,14株Victoria系B型毒株HA1基因之间的同源性为97.0%~99.9%。我省2008年分离的Yamagata系流感病毒与疫苗株间有5~7个变异,2009-2011年分离的Victoria系流感病毒与疫苗株间有5~7个变异,2012年分离的Yamagata系流感病毒与疫苗株间有1~4个变异,其中最多只有1个变异位于抗原决定簇。结论 2008-2012年江西省B型流感HA1基因未发生明显变异,WHO推荐的B型流感疫苗株对我省流行的B型流感具有保护作用。
[Abstract]:Objective to investigate the variation characteristics of HA1 gene of influenza B strain isolated from Jiangxi Province from 2008 to 2012.Methods Influenza virus was isolated from nasopharyngeal swabs of influenza-like cases in hospitals and suspected influenza cases, and nucleic acid was extracted from the isolated influenza B virus.The viral gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR), and the nucleotide sequence was sequenced. The sequencing results were analyzed with DNA Star5.0 Mage. The amino acid sequence was deduced and the gene characteristics were analyzed.Results the homology of HA1 gene among 10 strains of Yamagata strain B was 97.5%. The homology of HA1 gene among 14 strains of Victoria strain B was 97.0% and 99.9%.There were 5 ~ 7 mutations between Yamagata influenza viruses and vaccine strains isolated in 2008, 5 ~ 7 variants between Victoria influenza viruses and vaccine strains isolated in 2009-2011, and 1 ~ 4 variants between Yamagata influenza viruses and vaccine strains isolated in 2012.At most, only one of the variants was located in the antigenic determinant.Conclusion there was no significant variation in the HA1 gene of influenza B in Jiangxi Province from 2008 to 2012. The influenza B vaccine recommended by WHO has protective effect on the influenza type B epidemic in Jiangxi Province.
【作者单位】：江西省疾病预防控制中心;
【分类号】：R511.7

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