全自动微粒子化学发光免疫实验与TP-IgM抗体检测在梅毒检测中的应用效果比较

发布时间：2018-04-14 04:02

本文选题：梅毒 + 血清学检验　；参考：《中国性科学》2016年12期

【摘要】：目的:考察全自动微粒子化学发光免疫实验(CMIA)与TP-Ig M抗体检测(TP-ELISA)在梅毒检测中的应用效果差异。方法:采用TPPA法作为金标准检测1000例住院患者血清标准,评价CMIA与TP-ELISA在梅毒检测中敏感性和特异性。结果:金标准中1000例血清标本梅毒检出率为12.3%,CMIA阳性检出率为12.9%,TP-Ig M为11.7%。CMIA法灵敏度为98.4%(121/123),特异性为99.1%(869/877),阳性预测值为93.8%(121/129),阴性预测值为99.8%(869/871)。TP-ELISA法灵敏度为89.4%(110/123),特异性为99.2%(870/877),阳性预测值为94.0%(110/117),阴性预测值为98.5%(870/883)。两组间灵敏度和阴性预测值存在显著差异(P0.05)。结论:CMIA的灵敏性和阴性预测值优于TP-ELISA法,可以替代TP-PA成为梅毒检测的血清学检测首选方法。
[Abstract]:Objective: to investigate the difference of application effect between automatic microparticle chemiluminescence immunoassay (CMIA) and TP-Ig M antibody test (TP-ELISA) in syphilis detection.Methods: TPPA was used as the gold standard to detect the serum of 1000 inpatients, and the sensitivity and specificity of CMIA and TP-ELISA in syphilis detection were evaluated.Results: the positive detection rate of syphilis in 1000 serum samples of gold standard was 12.3CMIA. The sensitivity of TP-Ig M was 98.441 / 123N, the specificity was 99.1 / 869 / 877m, the positive predictive value was 93.881 / 129m, the negative predictive value was 89.41010 / 123U, the specificity was 99.270r877m, the specificity was 99.270r877m.The negative predictive value was 98.5 / 870 / 883.There was significant difference in sensitivity and negative predictive value between the two groups (P 0.05).ConclusionThe sensitivity and negative predictive value of CMIA are superior to that of TP-ELISA, and can replace TP-PA as the first choice for serological detection of syphilis.
【作者单位】：延安大学附属医院检验科;延安大学附属医院皮肤科;延安市人民医院;
【基金】：陕西省延安市科学技术研究发展计划项目(2014HM-07)
【分类号】：R759.1;R446.6

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