HIV-1两对重要蛋白质相互作用的研究

发布时间：2018-04-14 04:37

本文选题：Vpu-gp120 + gp41-Integrase　；参考：《华中农业大学》2013年硕士论文

【摘要】：艾滋病(Acquired Immune Deficiency Syndrome, AIDS)已经成为严重威胁人类健康的公共卫生问题。HIV(Human Immunodeficiency Virus)是引起艾滋病的病原。研究HIV蛋白间的相互作用及其与宿主细胞蛋白间的相互作用对于理解HIV的感染机制、生命周期等意义重大。本实验室在前期利用酵母双杂交技术系统地研究了HIV-1蛋白-蛋白相互作用,发现了10对蛋白间存在相互作用,其中有4对蛋白相互作用在以往的文献中未见报道。在此基础上,本论文对其中两对新发现的蛋白相互作用(Vpu-gp120; gp41-Integrase)进行了更深入的研究。本研究首先利用免疫共沉淀的方法检测到HIV-1Vpu蛋白和包膜糖蛋白gp120在生理条件下的相互作用,进一步证实了前期酵母双杂交的实验结果。随后对Vpu蛋白进行了一系列的截短和缺失,并利用酵母双杂交实验找到其与gp120蛋白相互作用的位点为Vpu蛋白72-81位氨基酸序列。同时,本研究也对包膜糖蛋白gp41进行了一系列的截短,通过酵母双杂交实验找到了其与整合酶Integrase相互作用的位点,即gp41蛋白的84-100位氨基酸序列。本研究发现了Vpu与gp120在生理条件下存在相互作用,并找到了Vpu与gp120及gp41与Integrase相互作用的位点,为探求这些相互作用的生物学意义提供了思路和方向,也为深入理解HIV-1的侵染机制等提供了重要的数据资料。
[Abstract]:HIV / AIDS Immune Deficiency Syndrome (AIDSs) has become a serious public health problem threatening human health. HIV Immunodeficiency virus is the cause of AIDS.It is important to study the interaction between HIV proteins and host cell proteins for understanding the mechanism and life cycle of HIV infection.The HIV-1 protein-protein interaction was systematically studied by yeast two-hybrid technique in our laboratory, and 10 pairs of proteins were found to interact with each other, of which 4 pairs were not reported in previous literatures.On this basis, two pairs of newly discovered protein interactions, Vpu-gp120; gp41-Integrase, were further studied in this paper.In this study, the interaction between HIV-1Vpu protein and envelope glycoprotein gp120 under physiological conditions was detected by immunoprecipitation, which further confirmed the experimental results of yeast two-hybrid.Then a series of truncation and deletion of Vpu protein were carried out, and the site of interaction with gp120 protein was found to be the amino acid sequence of Vpu protein at the 72-81 position by yeast two-hybrid experiment.At the same time, a series of truncation of envelope glycoprotein gp41 was carried out, and the site of interaction with integrase Integrase, the 84-100 amino acid sequence of gp41 protein, was found by yeast two-hybrid experiments.In this study, we found the interaction between Vpu and gp120 under physiological conditions, and found the sites where Vpu interacted with gp120 and gp41 and Integrase, which provided ideas and directions for exploring the biological significance of these interactions.It also provides important data for further understanding the infection mechanism of HIV-1.
【学位授予单位】：华中农业大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R512.91

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