重组人血管内皮抑素抑制沙鼠肝泡状棘球蚴周围血管生成的实验研究

发布时间：2018-04-18 08:20

本文选题：泡状棘球蚴 + 肝　；参考：《中国病原生物学杂志》2017年04期

【摘要】：目的探讨重组人血管内皮抑素恩度(endostar,YH-16)对沙鼠肝泡状棘球蚴(又称多房棘球蚴)组织周围血管生成的抑制作用。方法采用开腹肝穿刺法建立沙鼠肝泡球蚴动物模型,泡球蚴原头节混悬液接种量为0.1ml(约含原头节500个)。实验随机分为模型组(A组)、生理盐水组(B组)和重组人血管内皮抑素组(C组),10只/组。A组造模后不予任何干预,C组造模30d后开始给予恩度(10mg/kg)腹腔注射,1次/d,连用14d后停药7d,21d为一个疗程。B组腹腔注射等量生理盐水,方法同C组。用药3个疗程后解剖沙鼠,留取肝泡球蚴组织,采用免疫组化法和Western blot检测沙鼠肝泡状棘球蚴组织中血管内皮生长因子(VEGF)的表达及微血管密度(MVD-CD34)。结果 A、B、C组泡球蚴组织中VEGF评分分别为5.00±1.26、5.17±0.75、3.25±1.04,VEGF/β-肌动蛋白比值分别为1.11±0.06、1.10±0.07、0.63±0.10,C组VEGF表达水平与A、B组比较差异有统计学意义(P0.05);MVD-CD34计数分别为9.20±1.02、8.76±1.34、4.55±1.09,CD34/β-肌动蛋白比值分别为1.10±0.10、1.13±0.07、0.77±0.12,C组微血管计数及CD34蛋白表达量与A、B组比较差异有统计学意义(P0.05),A、B组相比VEGF和MVD-CD34表达水平差异无统计学意义(P0.05)。结论恩度干预肝泡球蚴小鼠VEGF表达及微血管密度下降,表明重组人血管内皮抑素可抑制泡状棘球蚴周围血管生成。
[Abstract]:Objective to investigate the inhibitory effect of recombinant human vascular endostatin Endostatin on angiogenesis in rat liver alveolar hydatid (Echinococcus multilocularis) tissue.Methods the liver alveolar hydatid model of gerbils was established by open liver puncture method. The inoculation amount of alveolar echinococci was 0.1 ml (about 500 pieces of original head ganglia).The experiment was randomly divided into two groups: group A (model group), group B (normal saline group) and group C (recombinant human vascular endostatin group). 10 rats in group A were injected intraperitoneally with Endostatin (10 mg / kg) after 30 days of model making without any intervention in group C (n = 10 mg / kg).After 14 days, the drug was stopped for 7 days and 21 days as a course of treatment. Group B was intraperitoneally injected with the same amount of normal saline.Methods same as group C.After three courses of administration, gerbils were dissected and liver alveolar hydatid tissues were collected. The expression of vascular endothelial growth factor (VEGF) and microvessel density (MVD-CD34) in the liver alveolar hydatid tissues of gerbils were detected by immunohistochemical method and Western blot.There was no significant difference in the expression of VEGF and MVD-CD34 between group A (P 0.05) and group A (P 0.05), and there was no significant difference between group A (P 0.05) and group A (P 0.05).Conclusion Endor interferes with the decrease of VEGF expression and microvessel density in mice with hepatic alveolar hydatid, indicating that recombinant human vascular endostatin can inhibit angiogenesis around echinococcus alveolaris.
【作者单位】：石河子大学医学院;石河子大学医学院第一附属医院肝胆外科;
【基金】：国家自然科学基金项目(No.81560518)
【分类号】：R532.32

【相似文献】