长非编码RNA在HIV-1感染单核细胞中的作用

发布时间：2018-04-20 07:26

本文选题：HIV + 单核细胞　；参考：《安徽医科大学学报》2017年05期

【摘要】：目的观察外周血单核细胞的长非编码RNA(lncRNA)在感染人类免疫缺陷病毒(HIV)-1前后的表达差异,并研究差异表达的lncRNAs在HIV-1感染单核细胞中的作用。方法采用基因芯片从HIV-1感染的单核细胞和未感染的对照单核细胞中筛选出表达差异最大的lncRNAs,并进行qRT-PCR验证。设计siRNA并转染到单核细胞中,ELISA检测转染siRNAs前后单核细胞表达白介素(IL)-1β、IL-10和肿瘤坏死因子(TNF)-α的变化。结果基因芯片和qRTPCR结果均显示,单核细胞感染HIV-1后,lncRNA-n266623表达显著上升;在转染lncRNA-n266623的siRNA后,单核细胞分泌IL-1β、IL-10和TNF-α的能力增强。结论 HIV感染单核细胞后能促进单核细胞高表达lncRNA-n266623;lncRNA-n266623可能抑制单核细胞的IL-1β、IL-10和TNF-α的分泌,从而负向调控机体对HIV-1感染的免疫应答。
[Abstract]:Objective to observe the expression of lncRNAs in peripheral blood monocytes before and after infection with HIV-1 and to investigate the role of differentially expressed lncRNAs in HIV-1 infected monocytes. Methods HIV-1 infected monocytes and uninfected monocytes were used to screen out the most differentially expressed lncRNAss by gene microarray, and the results were verified by qRT-PCR. SiRNA was designed and transfected into monocytes to detect the expression of IL-10 and TNF- 伪 in monocytes before and after transfection of siRNAs. Results the results of gene chip and qRTPCR showed that the expression of n266623 increased significantly after monocytes infected with HIV-1, and the ability of monocytes to secrete IL-1 尾 -IL-10 and TNF- 伪 was enhanced after transfection of siRNA with lncRNA-n266623. Conclusion HIV infection can promote the overexpression of lncRNA-n266623lncRNA-n266623lncRNA-n266623 in monocytes, which may inhibit the secretion of IL-1 尾 -IL-10 and TNF- 伪 in monocytes, thus negatively regulate the immune response of monocytes to HIV-1 infection.
【作者单位】：安徽医科大学病原生物学教研室;蚌埠医学院第一附属医院检验科;
【基金】：国家“973”计划项目(编号:2010CB530001)
【分类号】：R512.91

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