4-苯乙烯磺酸马来酸酐共聚物抑制HIV-1的初步研究

发布时间：2018-04-20 18:06

本文选题：-苯乙烯磺酸马来酸酐共聚物 + 抗病毒　；参考：《重庆医学》2015年01期

【摘要】：目的研究4-苯乙烯磺酸马来酸酐共聚物(PSM)对HIV-1的抑制作用。方法通过Luciferase检测不同浓度的PSM对易感细胞GHOST(3)X4/Hi5中HIV-1的抑制作用,观察PSM对北美HIV-1毒株JR-FL、HXB2和中国临床分离株CNE6、CNE30、CNE50和CNE55的抑制作用;利用CCK8试剂盒测定PSM对VK2/E6E7生长的抑制率来观察PSM的体外细胞毒性。通过逆转录荧光实时定量PCR(qRT-PCR)检测生殖道上皮细胞HEC-1-A的紧密连接蛋白ZO-1、E-cadherin和Occludin的表达,观察PSM对生殖道上皮细胞HEC-1-A表达的紧密连接蛋白的影响从而间接评价其对局部黏膜完整性的影响。结果PSM对北美HIV-1毒株JR-FL、HXB2以及中国常见临床分离株CNE6、CNE30、CNE50和CNE55的抑制EC50分别为5.78、0.77、1.85、3.15、1.70和2.27μg/mL,具有抗HIV-1感染的活性;PSM对VK2/E6E7细胞具有低毒性;PSM不明显抑制ZO-1的表达且增加了E-cadherin和Occludin的转录水平。结论 PSM可以抑制HIV-1感染,具有作为杀微生物剂的潜能。
[Abstract]:Objective to study the inhibitory effect of 4-styrene sulfonic acid maleic anhydride copolymer (PSM) on HIV-1. Methods the inhibitory effects of PSM at different concentrations on HIV-1 in susceptible cell GHOST(3)X4/Hi5 were detected by Luciferase, and the inhibitory effects of PSM on HIV-1 strain JR-FLN HXB2 and CNE6 CNE30 CNE50 and CNE55 were observed. The inhibition rate of PSM on VK2/E6E7 growth was determined by CCK8 kit to observe the cytotoxicity of PSM in vitro. The expression of HEC-1-A tight junction protein ZO-1E-cadherin and Occludin in reproductive tract epithelial cells was detected by reverse transcription-fluorescence real-time quantification of PCRQRT-PCR. To observe the effect of PSM on the expression of HEC-1-A tight junction protein in the epithelial cells of reproductive tract and evaluate indirectly its effect on the local mucosal integrity. Results the inhibitory EC50 of PSM on HIV-1 virus strain JR-FLHXB2 in North America and CNE6 CNE30 CNE50 and CNE55 on VK2/E6E7 cells were 5.787, 1.85, 3.151.70 and 2.27 渭 g / mL, respectively. PSM, which had anti- activity, did not inhibit the expression of ZO-1 and increased the transcription level of E-cadherin and Occludin in VK2/E6E7 cells. Conclusion PSM can inhibit HIV-1 infection and has the potential as a microbicide.
【作者单位】：江苏大学附属医院检验科;南京大学医学院;扬州市第一人民医院儿科;
【基金】：国家卫计委“十二五”传染病重大专项项目(2012ZX10001007-009) 江苏大学医学临床科技发展项目基金资助项目(JLY20120169)
【分类号】：R512.91

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