酶联荧光分析法在HIV感染筛查中的应用

发布时间：2018-04-21 06:20

本文选题：艾滋病病毒感染 + 酶联荧光分析法　；参考：《中国艾滋病性病》2017年10期

【摘要】：目的分析酶联荧光分析法(ELFA)检测艾滋病病毒(HIV)阳性反应样本与蛋白印迹试验(WB)确证结果的相关性,探讨ELFA在HIV感染筛查中的应用。方法回顾2014年6月至2016年3月,杭州市各筛查实验室上送样本中,ELFA检测HIV抗体阳性反应样本2 113例,HIVp24抗原阳性反应样本39例,用WB试验进行确证。比较ELFA阳性反应结果和WB确证结果的相关性,分析ELFA检测HIV抗体不同检测值对WB确证结果的预测性。结果 2 113例抗体阳性反应样本与WB试验结果符合率为98.25%(2 076例)。剔除46例无随访结果的样本,进一步讨论2 067例抗体阳性反应样本,ELFA法检测HIV抗体阳性预测值为97.92%(2 024例)。39例HIVp24抗原阳性反应样本首次WB确证36例(92.31%)为结果不确定,已随访的23例均确证为阳性。结论ELFA检测HIV抗体抗原结果直观,抗体阳性反应样本和WB确证试验有很好的相关性和预测性,是HIV抗体检测S/CO比值替代策略中一种较好的检测方法。
[Abstract]:Objective to analyze the correlation between the positive reaction samples of HIV (ELFA) and the results of Western blot test (WB), and to explore the application of ELFA in the screening of HIV infection. Methods from June 2014 to March 2016, the samples of the screening laboratories in Hangzhou were sampled and ELFA was used to detect the positive reaction samples of HIV antibody 2113 For example, 39 cases of HIVp24 antigen positive reaction were confirmed by WB test. The correlation between the results of ELFA positive reaction and the results of WB confirmed was compared. The predictability of WB confirmed results by different detection values of HIV antibody by ELFA was analyzed. Results the coincidence rate of the antibody positive reaction samples and WB test results was 98.25% (2076 cases). 46 cases were excluded from follow-up. The sample of 2067 cases of antibody positive reaction was further discussed. The predictive value of HIV antibody positive was 97.92% (2024 cases) by ELFA method (2024 cases) and 36 cases (92.31%) confirmed by WB confirmed for the first time (92.31%) for the positive reaction of HIVp24 antigen (92.31%), and all the 23 cases were confirmed to be positive. The result of HIV antibody antigen detection was intuitionistic and the antibody positive reaction was positive. The sample and WB confirmatory test have good correlation and predictability, which is a better method for HIV antibody detection in S/CO ratio replacement strategy.

【作者单位】：杭州市疾病预防控制中心;
【基金】：浙江省医药卫生科技计划项目(2015KYA189)~~
【分类号】：R512.91

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