追踪分析化学发光在低风险人群HCV感染确证中的应用

发布时间：2018-04-23 03:23

本文选题：化学发光微粒子免疫测定 + 重组免疫印迹试验　；参考：《中国输血杂志》2017年05期

【摘要】：目的通过追踪分析化学发光在低风险人群HCV感染确证中的应用。方法收集102例ELISA1抗-HCV反应性献血者及5例追踪血液样本、100例多次献血者阴性对照样本,补充另一种酶联免疫吸附试验(ELISA2)、重组免疫印迹试验(WB)、化学发光试验(CMIA)和HCV RNA定性检测,分析CMIA检测结果与HCV感染确证的关联。结果以ELISA2及WB法检测结果为参考标准,CMIA与参考标准结果高度相关(Spearman相关系数rs=0.866,P0.01),Kappa检验两者一致性极强(Kappa=0.857,P0.01)。追踪5例CMIA与参考标准不一致样本,5例ELISA2、WB、HCV RNA均为阴性,2例CMIA阳性,3例ELISA1阳性。结论 CMIA可以较好检出低风险人群确证HCV感染样本,但也存在一些假阳性。
[Abstract]:Objective to trace the application of chemiluminescence in the identification of HCV infection in low risk population. Methods 102 cases of ELISA1 anti -HCV reactive blood donors and 5 blood samples were collected, 100 negative control samples of multiple blood donors, another enzyme linked immunosorbent test (ELISA2), recombinant immunoblotting test (WB), chemiluminescence test (CMIA). The correlation between the results of CMIA detection and the confirmation of HCV infection was analyzed with HCV RNA. Results the results of ELISA2 and WB were the reference standard, and CMIA was highly correlated with the results of the reference standard (Spearman correlation coefficient rs=0.866, P0.01), and Kappa test was very consistent (Kappa= 0.857). ELISA2, WB, HCV RNA were all negative, 2 cases of CMIA positive, 3 cases of ELISA1 positive. Conclusion CMIA can be better identified in low risk population to confirm HCV infection samples, but there are some false positive.

【作者单位】：深圳市血液中心;
【基金】：深圳市科技创新委员会项目(JCYJ20140403093211510) 深圳市科卫生计生系统科研项目(201401074)
【分类号】：R512.63

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