江苏省连续3年HIV-1耐药警戒线及流行亚型的调查研究

发布时间：2018-04-23 18:54

本文选题：HIV- + 耐药警戒线调查　；参考：《南京医科大学学报(自然科学版)》2015年09期

【摘要】：目的 :了解江苏省艾滋病新近感染者在开展艾滋病抗病毒治疗以来HIV-1耐药株的传播水平及亚型分布情况。方法:采用WHO的截断序贯抽样法,连续3年在江苏省部分地区采集16~25岁HIV-1新近感染者的血样本各65、66和49例,提取病毒RNA,采用RT-PCR和巢式PCR扩增HIV-1pol区片段并测定pol区编码蛋白酶和逆转录酶基因的序列,提交到斯坦福大学HIV在线耐药数据库中鉴定耐药位点。用MEGA4.0软件构建进化树,分析亚型。结果:根据采样时间对样本排序,2009—2010年前47例未检出耐药样本数,2011年第28个序列出现1例耐药传播相关的耐药突变,后续一直到第44例均未有耐药样本出现,按传播水平判别表,属HIV耐药株的低度传播水平,采集的样本按pol区分型,2009、2010、2011年调查对象成功获得序列的前47例(2011年为44例)的亚型结果看,亚型以CRF01_AE为主,分别占40.40%、46.80%和56.80%,其次为CRF07_BC,分别为29.80%、25.50%、27.30%,其余还有CRF08_BC、C、B、URFA/B。结论:2009—2011年江苏省HIV-1新近感染者中HIV-1耐药株传播水平属低度,主要亚型流行情况与2006年本省调查结果类似,但与2003年前调查的HIV-1流行亚型比还是发生了显著变化。
[Abstract]:Objective: to investigate the transmission level and subtype distribution of HIV-1 resistant strains among newly infected AIDS patients in Jiangsu Province. Methods: blood samples of newly infected HIV-1 patients aged 16 ~ 25 years were collected in Jiangsu Province for 3 years by truncated sequential sampling method of WHO. RT-PCR and nested PCR were used to amplify the HIV-1pol region, and the sequences of protease and reverse transcriptase genes encoding pol region were sequenced. It was submitted to HIV online database of Stanford University for identification of drug resistance sites. The evolutionary tree was constructed with MEGA4.0 software and the subtypes were analyzed. Results: according to the sampling time, 47 cases of drug resistance samples were not detected before 2009-2010, 1 case of resistance mutation related to drug resistance transmission appeared in the 28th sequence in 2011, and no drug resistance samples appeared in the following cases until 44 cases. According to the discriminant table of transmission level, it belongs to the low transmission level of HIV resistant strains. The samples were collected according to pol discriminant type 2009 / 2010. The subtypes of the first 47 cases (44 cases in 2011) obtained successfully in 2011 showed that CRF01_AE was the main subtype. They accounted for 46.80% and 56.80% respectively, followed by CRF07BC, 29.80 and 25.50 and 27.30, respectively, and the rest in CRF08BCU / BURFA-B. Conclusion the transmission level of HIV-1 resistant strains among the newly infected individuals of HIV-1 in Jiangsu Province from 2009 to 2011 was low, and the prevalence of the main subtypes was similar to that of 2006, but it was significantly different from that of the HIV-1 subtypes investigated before 2003.
【作者单位】：江苏省疾病预防控制中心性病艾滋病防制所;
【基金】：江苏省医学重点人才项目(RC2011083,RC2011086,RC2011087)
【分类号】：R512.91

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